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Protective and regenerative effects of a novel medical device against esophageal mucosal damage using in vitro and ex vivo models
- Source :
- Biomedicine & Pharmacotherapy, Vol 131, Iss, Pp 110752-(2020)
- Publication Year :
- 2020
-
Abstract
- Gastroesophageal reflux disease (GERD) is a common digestive disorder that causes esophagitis and injuries to the esophageal mucosa. GERD symptoms are recurrent during pregnancy and their treatment is focused on lifestyle changes and nonprescription medicines. The aim of this study was to characterize the mechanism of action of a new patented medical device, an oral formulation containing hyaluronic acid, rice extract, and amino acids dispersed in a bioadhesive polymer matrix, by assessing its protective effects in in vitro and ex vivo models of esophageal mucosa damage. Acidic bile salts and pepsin cocktail (BSC) added to CP-A and COLO-680 N esophagus cells were used as an in vitro GERD model to evaluate the binding capacities, anti-inflammatory effects and reparative properties of the investigational product (IP) in comparison to a viscous control. Our results showed that the IP prevents cell permeability and tight junction dysfunction induced by BSC. Furthermore, the IP was also able to down-regulate IL-6 and IL-8 mRNA expression induced by BSC stimulation and to promote tissue repair and wound healing. The results were confirmed by ex vivo experiments in excised rat esophagi through the quantification of Evans Blue permeability assay. These experiments provided evidence that the IP is able to bind to the human esophagus cells, preventing the damage caused by gastroesophageal reflux, showing potential anti-irritative, soothing, and reparative properties.
- Subjects :
- 0301 basic medicine
Esophageal Mucosa
Hyaluronic acid
RM1-950
Pharmacology
Permeability
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Pepsin
Cell Line, Tumor
Digestive disorder
Medicine
Humans
Regeneration
Esophagus
Amino Acids
Hyaluronic Acid
Evans Blue
Medical device
biology
business.industry
Bioadhesive polymer
Gastroesophageal reflux disease (GERD)
Rice extract
Plant Extracts
Adhesiveness
Oryza
General Medicine
medicine.disease
digestive system diseases
030104 developmental biology
medicine.anatomical_structure
chemistry
Equipment and Supplies
030220 oncology & carcinogenesis
biology.protein
GERD
Gastroesophageal Reflux
Therapeutics. Pharmacology
business
Wound healing
Ex vivo
Subjects
Details
- ISSN :
- 19506007
- Volume :
- 131
- Database :
- OpenAIRE
- Journal :
- Biomedicinepharmacotherapy = Biomedecinepharmacotherapie
- Accession number :
- edsair.doi.dedup.....12abb68530bd854e89e4364949a51309