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Purification and characterization of a 1,3-β-D-glucan recognition protein from Antheraea pernyi larve that is regulated after a specific immune challenge
- Source :
- BMB Reports, Vol 46, Iss 5, Pp 264-269 (2013), BMB Reports
- Publication Year :
- 2013
- Publisher :
- Korean Society for Biochemistry and Molecular Biology, 2013.
-
Abstract
- Pattern recognition receptors are known to participate in the activation of Prophenoloxidase system. In this study, a 1,3-β-D-glucan recognition protein was detected for the first time in Antheraea pernyi larvae (Ap-βGRP). Ap-βGRP was purified to 99.9% homogeneity from the hemolymph using traditional chromatographic methods. Ap-βGRP specifically bind 1,3-β-D-glucan and yeast, but not E. coli or M. luteus. The 1,3-β-D-glucan dependent phenoloxidase (PO) activity of the hemolymph inhibited by anti-Ap-βGRP antibody could be recovered by addition of purified Ap-βGRP. These results demonstrate that Ap-βGRP acts as a biosensor of 1,3-β-Dglucan to trigger the Prophenoloxidase system. A trace mount of 1,3-β-D-glucan or Ap-βGRP alone was unable to trigger the proPO system, but they both did. Ap-βGRP was specifically degraded following the activation of proPO with 1,3-β-Dglucan. These results indicate the variation in the amount of Ap-βGRP after specific immune challenge in A. pernyi hemolymph is an important regulation mechanism to immune response. [BMB Reports 2013; 46(5): 264-269]
- Subjects :
- beta-Glucans
Molecular Sequence Data
Phenoloxidase (PO)
Antheraea pernyi
Biology
Moths
Biochemistry
lcsh:Biochemistry
Immune system
3-β-D-glucan recognition protein (βGRP)
Hemolymph
1,3-β-D-glucan recognition protein (βGRP)
Animals
Prophenoloxidase (proPO) system
lcsh:QD415-436
Amino Acid Sequence
Molecular Biology
lcsh:QH301-705.5
Research Articles
Innate immunity
Innate immune system
Sequence Homology, Amino Acid
Pattern recognition receptor
Histocompatibility Antigens Class II
General Medicine
Prophenoloxidase
biology.organism_classification
lcsh:Biology (General)
Larva
Immunology
biology.protein
Insect Proteins
Signal transduction
Antibody
Carrier Proteins
Sequence Alignment
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 19766696
- Volume :
- 46
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- BMB Reports
- Accession number :
- edsair.doi.dedup.....129cabfd36be635ff3f423c3cd667807