Additional file 2 of Optogenetically controlled human functional motor endplate for testing botulinum neurotoxins

Additional file 2: Figure S2. hMNs progenitors��� differentiation. a Schematic representation of the differentiation protocol based on Maury et al. (2015) to generate hMNs progenitors DIV 10. b Gene expression analysis in hMNs DIV 14 of relevant neuronal marker (MAP2), phenotypic markers (Islet1, AChE, ChAT), synaptic marker (SYP), and BoNT substrates (SNAP25, VAMP1, VAMP2, VAMP3) and receptors (SV2A, SV2B, SV2C, SYT1, SYT2). Expression is normalized to GAPDH and to control cDNA (human total adult spinal cord). c Immunofluorescence analysis of neuronal markers Islet1 and Tuj1 in hMNs DIV 14. Scale bars: 100 ��m.