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Bovine Sperm Sexing Alters Sperm Morphokinetics and Subsequent Early Embryonic Development
- Source :
- Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020), Scientific Reports
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- In artificial insemination the use of sex-sorted bovine sperm results in reduced conception, the causes of which are only partly understood. Therefore, we set out to investigate the effects of sexing on bovine sperm function and early embryonic development. Computer-assisted semen analysis (CASA) of sperm of the same bulls (n = 5), before and after sexing, demonstrated significantly reduced fast (A) and slow (B) progressively motile sperm (p in vitro produced embryos (n = 360), embryos derived from sexed-sperm displayed significantly increased incidences of arrest at the 4-cell stage (p
- Subjects :
- Male
0301 basic medicine
Embryology
endocrine system
medicine.medical_treatment
Embryonic Development
lcsh:Medicine
Cell Separation
Fertilization in Vitro
Sexing
Biology
Semen analysis
Time-Lapse Imaging
Article
Andrology
03 medical and health sciences
Pregnancy
medicine
Animals
Blastocyst
lcsh:Science
reproductive and urinary physiology
Cryopreservation
Microscopy, Video
Multidisciplinary
medicine.diagnostic_test
urogenital system
Artificial insemination
lcsh:R
Embryogenesis
0402 animal and dairy science
Embryo
04 agricultural and veterinary sciences
Embryo, Mammalian
Flow Cytometry
Oocyte
Spermatozoa
040201 dairy & animal science
Sperm
In Vitro Oocyte Maturation Techniques
Semen Analysis
030104 developmental biology
medicine.anatomical_structure
Oocytes
Cattle
Female
lcsh:Q
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 10
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....1298dfb68fea71f2a93ea5deb46f45d0
- Full Text :
- https://doi.org/10.1038/s41598-020-63077-6