Back to Search
Start Over
Super-resolution modularity analysis shows polyhedral caveolin-1 oligomers combine to form scaffolds and caveolae
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019)
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Caveolin-1 (Cav1), the coat protein for caveolae, also forms non-caveolar Cav1 scaffolds. Single molecule Cav1 super-resolution microscopy analysis previously identified caveolae and three distinct scaffold domains: smaller S1A and S2B scaffolds and larger hemispherical S2 scaffolds. Application here of network modularity analysis of SMLM data for endogenous Cav1 labeling in HeLa cells shows that small scaffolds combine to form larger scaffolds and caveolae. We find modules within Cav1 blobs by maximizing the intra-connectivity between Cav1 molecules within a module and minimizing the inter-connectivity between Cav1 molecules across modules, which is achieved via spectral decomposition of the localizations adjacency matrix. Features of modules are then matched with intact blobs to find the similarity between the module-blob pairs of group centers. Our results show that smaller S1A and S1B scaffolds are made up of small polygons, that S1B scaffolds correspond to S1A scaffold dimers and that caveolae and hemispherical S2 scaffolds are complex, modular structures formed from S1B and S1A scaffolds, respectively. Polyhedral interactions of Cav1 oligomers, therefore, leads progressively to the formation of larger and more complex scaffold domains and the biogenesis of caveolae.
- Subjects :
- 0301 basic medicine
Scaffold
Materials science
Caveolin 1
lcsh:Medicine
Coat protein
Caveolae
Article
Matrix decomposition
03 medical and health sciences
0302 clinical medicine
Image processing
Cell Line, Tumor
Humans
Molecule
Adjacency matrix
Super-resolution microscopy
lcsh:Science
030304 developmental biology
Microscopy
0303 health sciences
Modularity (networks)
Multidisciplinary
business.industry
Chemistry
lcsh:R
Cell Membrane
Modular design
Superresolution
Single Molecule Imaging
030104 developmental biology
030220 oncology & carcinogenesis
Biophysics
lcsh:Q
business
Biological system
030217 neurology & neurosurgery
Biogenesis
HeLa Cells
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....128498b1964696e2066b2fa24e288526