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Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
- Source :
- Journal of Visualized Experiments.
- Publication Year :
- 2020
- Publisher :
- MyJove Corporation, 2020.
-
Abstract
- Cancer stem cells play a vital role against clinical therapies, contributing to tumor relapse. There are many oncogenes involved in tumorigenesis and the initiation of cancer stemness properties. Since gene expression in the formation of colorectal cancer-derived tumorspheres is unclear, it takes time to discover the mechanisms working on one gene at a time. This study demonstrates a method to quickly discover the driver genes involved in the survival of the colorectal cancer stem-like cells in vitro. Colorectal HT29 cancer cells that express the LGR5 when cultured as spheroids and accompany an increase CD133 stemness markers were selected and used in this study. The protocol presented is used to perform RNAseq with available bioinformatics to quickly uncover the overexpressed driver genes in the formation of colorectal HT29-derived stem-like tumorspheres. The methodology can quickly screen and discover potential driver genes in other disease models.
- Subjects :
- 0301 basic medicine
Carcinogenesis
Colorectal cancer
General Chemical Engineering
Disease
Biology
medicine.disease_cause
General Biochemistry, Genetics and Molecular Biology
03 medical and health sciences
HT29 Cells
0302 clinical medicine
Cancer stem cell
Cell Line, Tumor
Spheroids, Cellular
medicine
Humans
General Immunology and Microbiology
General Neuroscience
LGR5
Cancer
medicine.disease
030104 developmental biology
030220 oncology & carcinogenesis
Cancer cell
Neoplastic Stem Cells
Cancer research
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 1940087X
- Database :
- OpenAIRE
- Journal :
- Journal of Visualized Experiments
- Accession number :
- edsair.doi.dedup.....1249a9c075b2c0a95ca9ed1300450676