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Monoclonal antibodies against rat glutathione S-transferase isoenzymes 2-2 and 3-3
- Source :
- Hybridoma 8 (1989), Hybridoma, 8, 475-480, Scopus-Elsevier
- Publication Year :
- 1989
-
Abstract
- Monoclonal antibodies were prepared against rat glutathione S-transferase isoenzymes 2-2 and 3-3. Three clones were capable of specifically differentiating their respective antigens from other rat isoenzymes as well as human isoenzymes, in ELISA and on Western blot. One clone produced antibodies specific for isoenzyme 2-2, and 2 hybridomas were specific for isoenzyme 3-3. Balb/c mice did not respond to immunization with glutathione S-transferase isoenzymes 1-1 and 4-4. However, an immune response was obtained in some other strains of mice, with differential H-2 haplotypes, notably CBA/BrARij mice and CBA/CaHRij-T6 mice for isoenzyme 1-1 and CBA/BrARij mice for isoenzyme 4-4, which offers perspectives for obtaining additional specific monoclonal antibodies against these glutathione S-transferases.
- Subjects :
- medicine.drug_class
Immunology
Mice, Inbred Strains
Cross Reactions
Monoclonal antibody
Mice
chemistry.chemical_compound
Western blot
Antigen
Antibody Specificity
Genetics
medicine
Animals
Life Science
Laboratorium voor Nematologie
Glutathione Transferase
Hybridomas
biology
medicine.diagnostic_test
Antibodies, Monoclonal
Glutathione
Molecular biology
Rats
Isoenzymes
Glutathione S-transferase
chemistry
Biochemistry
Monoclonal
biology.protein
Antibody
Laboratory of Nematology
Clone (B-cell biology)
Subjects
Details
- Language :
- English
- ISSN :
- 15540014
- Database :
- OpenAIRE
- Journal :
- Hybridoma 8 (1989), Hybridoma, 8, 475-480, Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....124937570c261fa093c51a604f4078ed