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Articular Cartilage Glycosaminoglycans Inhibit the Adhesion of Endothelial Cells
- Source :
- Connective Tissue Research. 53:220-228
- Publication Year :
- 2011
- Publisher :
- Informa UK Limited, 2011.
-
Abstract
- Articular cartilage undergoes severe loss of proteoglycan and its constituent glycosaminoglycans (GAGs) in osteoarthritis. We hypothesize that the low GAG content of osteoarthritic cartilage renders the tissue susceptible to pathological vascularization. This was investigated using an in vitro angiogenesis model assessing endothelial cell adhesion to GAG-depleted cartilage explants. Bovine cartilage explants were treated with hyaluronidase to deplete GAG content and then seeded with fluorescently tagged human endothelial cells (HMEC-1). HMEC-1 adherence was assessed after 4 hr and 7 days. The effect of hyaluronidase treatment on GAG content, chondrocyte viability, and biochemical composition of the extracellular matrix was also determined. Hyaluronidase treatment reduced the GAG content of cartilage explants by 78 ± 3% compared with that of controls (p < 0.0001). GAG depletion was associated with significantly more HMEC-1 adherence on both the surface (superficial zone) and the underside (deep zone) of the explants (both p < 0.0001). The latter provided a more favorable environment for extended culture of HMEC-1 compared with the articulating surface. Hyaluronidase treatment altered the immunostaining for chondroitin sulfate epitopes, but not for lubricin. Our results support the hypothesis that articular cartilage GAGs are antiadhesive to endothelial cells and suggest that chondroitin sulfate and/or hyaluronan are responsible. The loss of these GAGs in osteoarthritis may allow osteochondral angiogenesis resulting in disease progression.
- Subjects :
- Cartilage, Articular
Cell Survival
Angiogenesis
Hyaluronoglucosaminidase
Neovascularization, Physiologic
Osteoarthritis
Biochemistry
Chondrocyte
Extracellular matrix
Epitopes
chemistry.chemical_compound
Chondrocytes
Organ Culture Techniques
Rheumatology
Cell Adhesion
Human Umbilical Vein Endothelial Cells
medicine
Animals
Humans
Orthopedics and Sports Medicine
Chondroitin sulfate
Molecular Biology
Aggrecan
Glycoproteins
Glycosaminoglycans
biology
Chondroitin Sulfates
Cell Biology
medicine.disease
Bovine Cartilage
Cell biology
medicine.anatomical_structure
chemistry
Proteoglycan
Immunology
Disease Progression
biology.protein
Cattle
Female
Subjects
Details
- ISSN :
- 16078438 and 03008207
- Volume :
- 53
- Database :
- OpenAIRE
- Journal :
- Connective Tissue Research
- Accession number :
- edsair.doi.dedup.....123e7141545dc17070883d5b495f33a7