Detecting aquatic pathogens with field-compatible dried qPCR assays

Authors :: Jessica Rieder
Pedro M. Martin-Sanchez
Omneya A. Osman
Irene Adrian-Kalchhauser
Alexander Eiler
Norwegian Environment Agency
Rieder, Jessica [0000-0001-8922-9925]
Martin-Sanchez, Pedro Mª [0000-0003-2674-8650]
Adrian-Kalchhauser, Irene [0000-0002-7563-2577]
Eiler, Alexander [0000-0001-9916-9567]
Rieder, Jessica
Martin-Sanchez, Pedro Mª
Adrian-Kalchhauser, Irene
Eiler, Alexander
Source :: Digital.CSIC. Repositorio Institucional del CSIC, instname
Publication Year :: 2022
Publisher :: Cold Spring Harbor Laboratory, 2022.
Abstract: 4 páginas.- 2 figuras.- 1 tablas.- 15 referencias.- Supplementary data to this article can be found online at https://doi.org/10.1016/j.mimet.2022.106594<br />Field-ready qPCR assays with extended shelf-life support monitoring programs for emerging aquatic pathogens and enable quick conservation and management decisions. Here, we developed, validated, and tested the shelf-life of qPCR assays targeting Gyrodactylus salaris and Aphanomyces astaci with lyophilization and air-drying. © 2022 The Authors<br />This work was supported by the Norwegian Environment Agency (Auto e-DNA project).