Additional file 2 of PilG and PilH antagonistically control flagellum-dependent and pili-dependent motility in the phytopathogen Xanthomonas campestris pv. campestris

Additional file 2:Figure S2. PilH positively regulates EPS production but PilG not, and they have no effect on the growth in NY medium. (A) Analysis of EPS production on plates. The strains were grown and diluted to OD600 of 1, and then 3 μl diluted culture was inoculated onto the NY plates containing 2% glucose and 2.0% agar. The results were observed after 5 days of incubation at 28 °C. (B) Production of EPS in Xcc strains. Mean weight of EPS extracted from the wild type strain, the pilG mutant, the pilH mutant and the corresponding complemented strains. (C) Growth curve of Xcc strains in NY medium. The strains were inoculated into NYG medium with the same final density of 0.01, growth of the strains was diluted and plated on NYG plates at intervals of 4 h. The living cells were counted after 3 days of incubation at 28 °C. Significance was tested by Student’s t test (* and ** represent significance at P