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Decreased Metabolic Flexibility in Skeletal Muscle of Rat Fed with a High-Fat Diet Is Recovered by Individual CLA Isomer Supplementation via Converging Protective Mechanisms
- Source :
- Cells, Vol 9, Iss 823, p 823 (2020), Cells, Volume 9, Issue 4, Cells (2020). doi:10.3390/cells9040823, info:cnr-pdr/source/autori:Trinchese G, Cavaliere G, Cimmino F, Catapano A, Carta G, Pirozzi C, Murru E, Lama A, Meli R, Bergamo P, Banni S, Mollica MP./titolo:Decreased Metabolic Flexibility in Skeletal Muscle of Rat Fed with a High-Fat Diet Is Recovered by Individual CLA Isomer Supplementation via Converging Protective Mechanisms./doi:10.3390%2Fcells9040823/rivista:Cells/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Publication Year :
- 2020
-
Abstract
- Energy balance, mitochondrial dysfunction, obesity, and insulin resistance are disrupted by metabolic inflexibility while therapeutic interventions are associated with improved glucose/lipid metabolism in skeletal muscle. Conjugated linoleic acid mixture (CLA) exhibited anti-obesity and anti-diabetic effects<br />however, the modulatory ability of its isomers (cis9, trans11, C9<br />trans10, cis12, C10) on the metabolic flexibility in skeletal muscle remains to be demonstrated. Metabolic inflexibility was induced in rat by four weeks of feeding with a high-fat diet (HFD). At the end of this period, the beneficial effects of C9 or C10 on body lipid content, energy expenditure, pro-inflammatory cytokines, glucose metabolism, and mitochondrial efficiency were examined. Moreover, oxidative stress markers, fatty acids, palmitoyletanolamide (PEA), and oleyletanolamide (OEA) contents along with peroxisome proliferator-activated receptors-alpha (PPAR&alpha<br />), AKT, and adenosine monophosphate-activated protein kinase (AMPK) expression were evaluated in skeletal muscle to investigate the underlying biochemical mechanisms. The presented results indicate that C9 intake reduced mitochondrial efficiency and oxidative stress and increased PEA and OEA levels more efficiently than C10 while the anti-inflammatory activity of C10, and its regulatory efficacy on glucose homeostasis are associated with modulation of the PPAR&alpha<br />/AMPK/pAKT signaling pathway. Our results support the idea that the dissimilar efficacy of C9 and C10 against the HFD-induced metabolic inflexibility may be consequential to their ability to activate different molecular pathways.
- Subjects :
- Male
0301 basic medicine
Conjugated
medicine.disease_cause
metabolic flexibility
0302 clinical medicine
Glucose homeostasis
Homeostasis
Linoleic Acids, Conjugated
lcsh:QH301-705.5
Glucose Transporter Type 4
Chemistry
Fatty Acids
CLA
mitochondrial function
General Medicine
Muscle Skeletal
Mitochondria
medicine.anatomical_structure
Liver
Linoleic Acids
lipids (amino acids, peptides, and proteins)
CLA, metabolic flexibility, mitochondrial function, Adenylate Kinase, Animals, Energy Metabolism, Fatty Acids, Homeostasis, Inflammation, Linoleic Acids, Conjugated, Mitochondria , Muscle Skeletal, Proto-Oncogene Proteins c-akt, Feeding Behavior
Oxidation-Reduction
medicine.medical_specialty
Carbohydrate metabolism
Diet, High-Fat
Protective Agents
Article
03 medical and health sciences
Insulin resistance
Isomerism
Internal medicine
medicine
Animals
PPAR alpha
Rats, Wistar
Muscle, Skeletal
Protein kinase B
Inflammation
Adenylate Kinase
AMPK
Skeletal muscle
Lipid metabolism
Feeding Behavior
Lipid Metabolism
medicine.disease
Oxidative Stress
Glucose
030104 developmental biology
Endocrinology
lcsh:Biology (General)
Dietary Supplements
Energy Metabolism
Proto-Oncogene Proteins c-akt
030217 neurology & neurosurgery
Oxidative stress
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Cells, Vol 9, Iss 823, p 823 (2020), Cells, Volume 9, Issue 4, Cells (2020). doi:10.3390/cells9040823, info:cnr-pdr/source/autori:Trinchese G, Cavaliere G, Cimmino F, Catapano A, Carta G, Pirozzi C, Murru E, Lama A, Meli R, Bergamo P, Banni S, Mollica MP./titolo:Decreased Metabolic Flexibility in Skeletal Muscle of Rat Fed with a High-Fat Diet Is Recovered by Individual CLA Isomer Supplementation via Converging Protective Mechanisms./doi:10.3390%2Fcells9040823/rivista:Cells/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume
- Accession number :
- edsair.doi.dedup.....11fca256448cceb81a113b8e56b7af0e