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Evaluation of DNA extraction methods to detect bacterial targets in aerosol samples
- Source :
- Journal of Microbiological Methods. 153:48-53
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- DNA-based monitoring of pathogens in aerosol samples requires extraction methods that provide high recovery of DNA. To identify a suitable method, we evaluated six DNA extraction methods for recovery of target-specific DNA from samples with four bacterial agents at low abundance (
- Subjects :
- DNA, Bacterial
0301 basic medicine
Microbiology (medical)
Yersinia pestis
Polymerase Chain Reaction
Microbiology
Genome
Dynabeads
03 medical and health sciences
chemistry.chemical_compound
Francisella tularensis
Molecular Biology
Aerosols
Spores, Bacterial
Chromatography
Bacteria
biology
Chemistry
Burkholderia pseudomallei
biology.organism_classification
DNA extraction
Microspheres
Bacillus anthracis
030104 developmental biology
Magnets
Cell disruption
DNA
Subjects
Details
- ISSN :
- 01677012
- Volume :
- 153
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiological Methods
- Accession number :
- edsair.doi.dedup.....11df51c32ca8dfc463ce06f7c722cdf2
- Full Text :
- https://doi.org/10.1016/j.mimet.2018.09.006