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Comparative analysis ofCampylobacter laricytolethal distending toxin (CDT) effect on HeLa cells
- Source :
- Journal of Basic Microbiology. 52:559-565
- Publication Year :
- 2012
- Publisher :
- Wiley, 2012.
-
Abstract
- We aimed to clarify if Campylobacter lari exerts a cytolethal distending toxin (CDT) effect on HeLa cells. Campylobacter cell lysates (CCLys) from C. jejuni 81–176 and urease-positive thermophilic Campylobacter (UPTC) CF89-12 and UPTC NCTC12893 isolates were shown to exert a CDT effect on HeLa cells with morphological changes examined by Giemsa staining and microscopy. However, Campylobacter lari JCM2530T isolate showed no effect. In addition, Campylobacter cell culture supernatant wash gave low or absent toxic effects with both C. jejuni and C. lari organisms. When western blot analysis was carried out to clarify if there was a CDTB effect in the CCLys and soluble fractions from Campylobacter isolates, which had a CDT effect on HeLa cells or did not have any effect, anti-recombinant CjCDTB antibodies identified an immunoreactively positive signal at around approximately 25 kDa on all the C. lari isolates examined, as well as the C. jejuni 81116 strain. Thus, all the Campylobacter isolates including those without any CDT effect were shown to express CDTB at the translational level. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)
- Subjects :
- Microscopy
biology
medicine.diagnostic_test
Cytolethal distending toxin
Campylobacter
Bacterial Toxins
Blotting, Western
Lari
Campylobacter lari
Epithelial Cells
General Medicine
biology.organism_classification
medicine.disease_cause
Applied Microbiology and Biotechnology
Giemsa stain
Microbiology
HeLa
Western blot
biology.protein
medicine
Humans
Antibody
HeLa Cells
Subjects
Details
- ISSN :
- 0233111X
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Journal of Basic Microbiology
- Accession number :
- edsair.doi.dedup.....11c7e0904240f78efd4ca0a48e33b1a7
- Full Text :
- https://doi.org/10.1002/jobm.201100297