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Comparative analysis ofCampylobacter laricytolethal distending toxin (CDT) effect on HeLa cells

Authors :
T. Nakajima
Masahiro Asakura
Motoo Matsuda
J. Hirayama
John E. Moore
Shinji Yamasaki
E. Tasaki
K. Hayashi
B.C. Millar
A. Tazumi
Source :
Journal of Basic Microbiology. 52:559-565
Publication Year :
2012
Publisher :
Wiley, 2012.

Abstract

We aimed to clarify if Campylobacter lari exerts a cytolethal distending toxin (CDT) effect on HeLa cells. Campylobacter cell lysates (CCLys) from C. jejuni 81–176 and urease-positive thermophilic Campylobacter (UPTC) CF89-12 and UPTC NCTC12893 isolates were shown to exert a CDT effect on HeLa cells with morphological changes examined by Giemsa staining and microscopy. However, Campylobacter lari JCM2530T isolate showed no effect. In addition, Campylobacter cell culture supernatant wash gave low or absent toxic effects with both C. jejuni and C. lari organisms. When western blot analysis was carried out to clarify if there was a CDTB effect in the CCLys and soluble fractions from Campylobacter isolates, which had a CDT effect on HeLa cells or did not have any effect, anti-recombinant CjCDTB antibodies identified an immunoreactively positive signal at around approximately 25 kDa on all the C. lari isolates examined, as well as the C. jejuni 81116 strain. Thus, all the Campylobacter isolates including those without any CDT effect were shown to express CDTB at the translational level. (© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim)

Details

ISSN :
0233111X
Volume :
52
Database :
OpenAIRE
Journal :
Journal of Basic Microbiology
Accession number :
edsair.doi.dedup.....11c7e0904240f78efd4ca0a48e33b1a7
Full Text :
https://doi.org/10.1002/jobm.201100297