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HIV transmission from infected CD4+ T cells to allogenic T and dendritic cells is inhibited by broadly neutralizing antibodies
- Source :
- AIDS (London, England). 32(10)
- Publication Year :
- 2018
-
Abstract
- Objective In the semen, both free virus and infected cells are able to establish HIV infection during sexual intercourse. An efficient vaccine should therefore inhibit both infectious states. The aim of this study was to analyze the capacity of broadly neutralizing antibodies (bNAbs) to inhibit HIV transmission by the infected cells. Design/methods We developed an in-vitro model aiming to mimic mucosal HIV transmission via infected cells. PHA-activated CD4+ T cells stained with PKH26 from donor A were infected and co-cultured with CD4+ T cells and dendritic cells from donor B in the presence of bNAbs. Results We showed that dendritic cells were the preferential HIV target cells at early time points in this co-culture model. In the context of this co-culture model where infection and transmission occurred simultaneously, bNAbs efficiently inhibited HIV replication as well as HIV transmission from infected cells to allogenic dendritic cells and CD4+ T cells. Conclusion Overall, our results indicate that dendritic cells, in addition to CD4+ T cells, are key cells that are efficiently infected by HIV and bNAbs are potent inhibitors of infection of both target cells. Future HIV prophylactic vaccine design should develop immune strategies able to prevent the infection of dendritic cells, in addition to the inhibition of CD4+ T-cell infection.
- Subjects :
- 0301 basic medicine
CD4-Positive T-Lymphocytes
Immunology
Human immunodeficiency virus (HIV)
Context (language use)
Semen
Blood Donors
HIV Infections
Biology
HIV Antibodies
medicine.disease_cause
Models, Biological
Virus
03 medical and health sciences
Immune system
medicine
Disease Transmission, Infectious
Immunology and Allergy
Humans
Hiv transmission
Cells, Cultured
Transmission (medicine)
Dendritic Cells
Virology
Antibodies, Neutralizing
Coculture Techniques
030104 developmental biology
Infectious Diseases
biology.protein
Antibody
Subjects
Details
- ISSN :
- 14735571
- Volume :
- 32
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- AIDS (London, England)
- Accession number :
- edsair.doi.dedup.....11c73f23751e83957b2ae0eba2417d25