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Rapid Identification of Microorganisms from Positive Blood Culture by MALDI-TOF MS After Short-Term Incubation on Solid Medium
- Source :
- Current microbiology. 74(1)
- Publication Year :
- 2016
-
Abstract
- Matrix-assisted laser-desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) is a useful tool for rapid identification of microorganisms. Unfortunately, its direct application to positive blood culture is still lacking standardized procedures. In this study, we evaluated an easy- and rapid-to-perform protocol for MALDI-TOF MS direct identification of microorganisms from positive blood culture after a short-term incubation on solid medium. This protocol was used to evaluate direct identification of microorganisms from 162 positive monomicrobial blood cultures; at different incubation times (3, 5, 24 h), MALDI-TOF MS assay was performed from the growing microorganism patina. Overall, MALDI-TOF MS concordance with conventional methods at species level was 60.5, 80.2, and 93.8% at 3, 5, and 24 h, respectively. Considering only bacteria, the identification performances at species level were 64.1, 85.0, and 94.1% at 3, 5, and 24 h, respectively. This protocol applied to a commercially available MS typing system may represent, a fast and powerful diagnostic tool for pathogen direct identification and for a promptly and pathogen-driven antimicrobial therapy in selected cases.
- Subjects :
- 0301 basic medicine
Microorganism
030106 microbiology
Bacteremia
Biology
Mass spectrometry
Microbiology
Applied Microbiology and Biotechnology
03 medical and health sciences
Tandem Mass Spectrometry
medicine
Bioassay
Matrix-Assisted Laser Desorption-Ionization
Humans
Blood culture
Bacteria
Biological Assay
Blood
Blood Culture
Culture Media
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Incubation
medicine.diagnostic_test
Spectrometry
General Medicine
Mass
Antimicrobial
biology.organism_classification
Matrix-assisted laser desorption/ionization
030104 developmental biology
Subjects
Details
- ISSN :
- 14320991
- Volume :
- 74
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Current microbiology
- Accession number :
- edsair.doi.dedup.....11c2b045d53e203ba0a2f143a7632072