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The effects of exogenous sphingosine on Neuro2a cells are strictly related to the overall capacity of cells to metabolize sphingosine
- Source :
- Scopus-Elsevier, ResearcherID
- Publication Year :
- 1998
-
Abstract
- Neuro2a cells were exposed to different doses (1-40 nmol/10(6) cells) of [C3-3H]sphingosine and the relationship between metabolism and biological effects of sphingosine was investigated. Sphingosine appeared to be rapidly taken up and metabolized. The incorporation of sphingosine was not merely dependent on its concentration but primarily on the dose per cell of administered sphingosine. At low doses, [3H]sphingosine represented a minor portion of the cellular radioactivity, and N-acylated metabolites, particularly ceramide, largely prevailed over degradation products. Concomitantly with ceramide increase, Neuro2a differentiation took place. With increasing exogenous sphingosine/doses, the acylation process reached saturation. From this point on, [3H]sphingosine started accumulating and eventually cell toxicity occurred. In conclusion, the biological effects exerted by exogenous sphingosine on Neuro2a cells are not merely dependent on the long-chain base concentration in the culture medium, but are strictly related to the cellular dose of exogenous sphingosine and to the capacity of cells to metabolize sphingosine.
- Subjects :
- Neurons
Ceramide
Sphingosine
Cellular differentiation
Cell
Cell Differentiation
General Medicine
Metabolism
Lipid signaling
medicine.disease
Ceramides
Biochemistry
Acylation
chemistry.chemical_compound
Mice
Neuroblastoma
medicine.anatomical_structure
chemistry
medicine
Tumor Cells, Cultured
Animals
Molecular Biology
Subjects
Details
- ISSN :
- 0021924X
- Volume :
- 124
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of biochemistry
- Accession number :
- edsair.doi.dedup.....11beb69cd1fdfb3a3a1e37f22dd735bb