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Glucagon-like peptide 1 increases glucose-dependent activity of the homeoprotein IDX-1 transactivating domain in pancreatic beta-cells
- Source :
- Biochemical and biophysical research communications. 274(3)
- Publication Year :
- 2000
-
Abstract
- Both glucose and glucagon-like peptide 1 (GLP-1) stimulate insulin gene transcription in endocrine pancreatic beta-cells within the islets of Langerhans. The effects of glucose are mediated by the homeodomain transcription factor islet duodenum homeobox -1 (IDX-1) that binds to two adenine thymidine-rich (A1 and A2/3) motifs within the rat insulin promoter. Glucose stimulates the activity of the transactivation domain of IDX-1 that lies within the first 80 amino acids of the IDX-1 protein. The effects of GLP-1 on insulin gene expression are primarily conferred by the cAMP responsive element (CRE) within the insulin promoter. GLP-1 stimulates glucose-dependent insulin release from beta-cells. We hypothesize that GLP-1 may augment the effects of glucose on insulin gene transcription. Here we show that GLP-1 stimulates insulin gene transcription independent of the CRE and is glucose-dependent. Furthermore, we show that GLP-1 stimulates the transactivational activity of IDX-1.
- Subjects :
- Transcriptional Activation
endocrine system
medicine.medical_specialty
medicine.medical_treatment
EMX2
Biophysics
Transfection
Biochemistry
Cell Line
Transactivation
Islets of Langerhans
Glucagon-Like Peptide 1
Insulin receptor substrate
Internal medicine
medicine
Animals
Protein Precursors
Molecular Biology
Transcription factor
Homeodomain Proteins
biology
Insulin
digestive, oral, and skin physiology
Cell Biology
TCF4
Glucagon
Peptide Fragments
Rats
Insulin receptor
Endocrinology
Glucose
biology.protein
Trans-Activators
PAX4
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 274
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....11a30b0a1d58605405124698e3f9cb0f