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Cell-free synthesis system-assisted pathway bottleneck diagnosis and engineering in Bacillus subtilis
- Source :
- Synthetic and Systems Biotechnology, Synthetic and Systems Biotechnology, Vol 5, Iss 3, Pp 131-136 (2020)
- Publication Year :
- 2020
- Publisher :
- KeAi Publishing, 2020.
-
Abstract
- Metabolic engineering is a key technology for cell factories construction by rewiring cellular resources to achieve efficient production of target chemicals. However, the existence of bottlenecks in synthetic pathway can seriously affect production efficiency, which is also one of the core issues for metabolic engineers to solve. Therefore, developing an approach for diagnosing potential metabolic bottlenecks in a faster and simpler manner is of great significance to accelerate cell factories construction. The cell-free reaction system based on cell lysates can transfer metabolic reactions from in vivo to in vitro, providing a flexible access to directly change protein and metabolite variables, thus provides a potential solution for rapid identification of bottlenecks. Here, bottleneck diagnosis of the N-acetylneuraminic acid (NeuAc) biosynthesis pathway in industrially important chassis microorganism Bacillus subtilis was performed using cell-free synthesis system. Specifically, a highly efficient B. subtilis cell-free system for NeuAc de novo synthesis was firstly constructed, which had a 305-fold NeuAc synthesis rate than that in vivo and enabled fast pathway dynamics analysis. Next, through the addition of all potential key intermediates in combination with substrate glucose respectively, it was found that insufficient phosphoenolpyruvate supply was one of the NeuAc pathway bottlenecks. Rational in vivo metabolic engineering of NeuAc-producing B. subtilis was further performed to eliminate the bottleneck. By down-regulating the expression level of pyruvate kinase throughout the growth phase or only in the stationary phase using inhibitory N-terminal coding sequences (NCSs) and growth-dependent regulatory NCSs respectively, the maximal NeuAc titer increased 2.0-fold. Our study provides a rapid method for bottleneck diagnosis, which may help to accelerate the cycle of design, build, test and learn cycle for metabolic engineering.
- Subjects :
- 0106 biological sciences
lcsh:Biotechnology
Biomedical Engineering
Computational biology
Bacillus subtilis
01 natural sciences
Applied Microbiology and Biotechnology
Bottleneck
Article
Metabolic engineering
03 medical and health sciences
chemistry.chemical_compound
Biosynthesis
Structural Biology
In vivo
010608 biotechnology
lcsh:TP248.13-248.65
Genetics
Cell-free synthesis system
lcsh:QH301-705.5
030304 developmental biology
0303 health sciences
biology
food and beverages
biology.organism_classification
Pathway bottleneck diagnosis
De novo synthesis
chemistry
lcsh:Biology (General)
Phosphoenolpyruvate carboxykinase
Pyruvate kinase
Subjects
Details
- Language :
- English
- ISSN :
- 2405805X
- Volume :
- 5
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Synthetic and Systems Biotechnology
- Accession number :
- edsair.doi.dedup.....118c8f4ffbf3261988e8bf4981b6a290