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Lansoprazole induces apoptosis of breast cancer cells through inhibition of intracellular proton extrusion
- Source :
- Biochemical and Biophysical Research Communications. 448:424-429
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- The increased glycolysis and proton secretion in tumors is proposed to contribute to the proliferation and invasion of cancer cells during the process of tumorigenesis and metastasis. Here, treatment of human breast cancer cells with proton pump inhibitor (PPI) lansoprazole (LPZ) induces cell apoptosis in a dose-dependent manner. In the implantation of the MDA-MB-231 xenografts in nude mice, administration of LPZ significantly inhibits tumorigenesis and induces large-scale apopotosis of tumor cells. LPZ markedly inhibits intracellular proton extrusion, induces an increase in intracellular ATP level, lysosomal alkalinization and accumulation of reactive oxygen species (ROS) in breast cancer cells. The ROS scavenger N-acetyl-l-cysteine (NAC) and diphenyleneiodonium (DPI), a specific pharmacological inhibitor of NADPH oxidases (NOX), significantly abolish LPZ-induced ROS accumulation in breast cancer cells. Our results suggested that LPZ may be used as a new therapeutic drug for breast tumor.
- Subjects :
- medicine.medical_specialty
Intracellular pH
Biophysics
Mice, Nude
Apoptosis
Breast Neoplasms
Biology
medicine.disease_cause
Biochemistry
Metastasis
Mice
Adenosine Triphosphate
Cell Line, Tumor
Internal medicine
medicine
Animals
Humans
Lansoprazole
Molecular Biology
chemistry.chemical_classification
Reactive oxygen species
Cell growth
Proton Pump Inhibitors
Cell Biology
Hydrogen-Ion Concentration
medicine.disease
Xenograft Model Antitumor Assays
Endocrinology
chemistry
Cancer cell
Cancer research
Female
Protons
Lysosomes
Reactive Oxygen Species
Carcinogenesis
Intracellular
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 448
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....1173dd9ffb3cbcae333059ea2c3d4f19