Back to Search
Start Over
Effects of platelet-rich plasma against experimental ischemia/reperfusion injury in rat testis
- Source :
- Journal of pediatric urology. 13(3)
- Publication Year :
- 2016
-
Abstract
- WOS: 000406687000043<br />PubMed ID: 28215833<br />Background Testicular torsion is a common problem and, to date, there is no agent to preserve testicular function following detorsion. Platelet-rich plasma (PRP), with its rich growth factor composition, has proven beneficial in regenerative therapy. It is believed that PRP has not been studied in testis for ischemia/ reperfusion (I/R) injury. Objective This study investigated the effect of PRP in an I/R rat model 1 month after detorsion. Study design Of 24 adult male Spraguee-Dawley rats, 18 were randomly assigned into three groups, with six in each: control, I/R and I/R + PRP. The PRP was prepared from the remaining six. Each group underwent right orchiectomy. Ischemia was performed by rotating the left testis 720 degrees and fixing with a nylon suture for 4 h. Reperfusion occurred 4 h later by removing the suture, and PRP was administered at a dose of 10 ml (2000 x 10(9)/l) into the left testis via the intra-parenchymal route. Animals were sacrificed at the fourth week, and testes were taken for malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), myeloperoxidase (MPO), transforming growth factor beta (TGF-beta), and caspase-3 measurements. Results Ischemia/reperfusion caused a significant increase in MDA, MPO and caspase-3 activity, and significant decrease in GSH levels and SOD activity. The PRP treatment helped correct the alterations in SOD, caspase-3, and MPO activities and MDA levels. However, the mean MDA level and MPO activity were not totally restored compared with the controls. Serum testosterone levels of the I/R group were significantly lower compared with the control and I/R + PRP groups. TGF-b and caspase-3 protein expressions were significantly higher in the I/R group compared with the control group and were low with PRP administration compared with I/R groups (summary Table). Discussion The findings of the present study suggest that PRP, by inhibiting neutrophil infiltration and oxidative stress and increasing antioxidant defense, exerts protective effects on testicular tissues against I/R. This study had some limitations: a scoring system was not used in the assessment of spermatogenesis in the histopathological findings and specific testis cell types were not histologically assessed. Conclusions In light of the biochemical, histological and, especially, hormonal findings, intraparenchymal PRP injection may have a protective effect in testicular tissue against I/R injury.
- Subjects :
- Male
medicine.medical_specialty
Testicular torsion
Urology
030232 urology & nephrology
Ischemia/reperfusion
Superoxide dismutase
Rats, Sprague-Dawley
Platelet-rich-plasma
03 medical and health sciences
chemistry.chemical_compound
Follicle-stimulating hormone
0302 clinical medicine
Transforming Growth Factor beta
Internal medicine
Medicine
Animals
Testosterone
Spermatic Cord Torsion
Inflammation
biology
business.industry
Caspase 3
Platelet-Rich Plasma
Glutathione
Malondialdehyde
medicine.disease
Hormones
Rats
Oxidative Stress
Endocrinology
chemistry
030220 oncology & carcinogenesis
Platelet-rich plasma
Myeloperoxidase
Reperfusion Injury
Pediatrics, Perinatology and Child Health
biology.protein
business
Luteinizing hormone
Reperfusion injury
Subjects
Details
- ISSN :
- 18734898
- Volume :
- 13
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of pediatric urology
- Accession number :
- edsair.doi.dedup.....11670aa428cd02b3358f0d43cc62e2a9