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Pericellular-acting proteases in human first trimester decidua
- Source :
- Molecular Human Reproduction, 1, 14, 41-51, Plaisier, M, Koolwijk, P, Willems, F, Helmerhorst, F M & van Hinsbergh, V W M 2008, ' Pericellular-acting proteases in human first trimester decidua ', Molecular Human Reproduction, vol. 14, no. 1, pp. 41-51 . https://doi.org/10.1093/molehr/gam085, Molecular Human Reproduction, 14(1), 41-51. Oxford University Press
- Publication Year :
- 2008
- Publisher :
- Oxford University Press (OUP), 2008.
-
Abstract
- Proteolysis is essential for decidual development during embryonic implantation, but little is known regarding the expression and functions of membrane-type matrix metalloproteinases (MT-MMPs) and urokinase-type plasminogen activator (uPA) and its receptor uPAR in decidua. Therefore, their protein and mRNA levels were analysed in three first trimester decidual tissues, decidual secretory endometrium (DSE), decidua parietalis (DP) and basalis (DB). Decidua was obtained during first trimester pregnancy termination. uPA, uPAR, and MT1/2/3/5-MMP expression were studied by RT-PCR and immunohistochemistry, and CD56-positive uNK cells and CD68-positive macrophages were quantified in serial sections. The mRNAs and antigens of all proteases and uPAR were detectable in the decidual tissues and extravillous trophoblasts (EVT). mRNA levels of all proteases and uPAR, except MT5-MMP, were elevated in both DB and DP compared to DSE, being significant for MT1-MMP and uPAR in DP. MT2- and MT3-MMP mRNAs in DB were 24- and 10-fold higher than in DSE, and 19- and 7-fold increased compared to DP. At the protein level uPA and uPAR were particularly elevated in DB, while pro-angiogenic MT1- and MT3-MMPs were elevated in both DB and DP compared to DSE. MT2-MMP was prominently present in all conditions. The number of uNK cells was increased in DB and DP versus DSE, while a comparable increase in macrophages did not reach statistical significance. These data are consistent with a differential regulation of pericellular proteases in decidua by pregnancy-induced hormones, immune cells and EVT. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
- Subjects :
- First trimester
Embryology
Biomedical Research
Decidua Parietalis
urokinase
CD68 antigen
Matrix metalloproteinase
Pregnancy
cell count
pathophysiology
quantitative analysis
messenger RNA
Reverse Transcriptase Polymerase Chain Reaction
adult
Decidua
Trophoblast
article
Obstetrics and Gynecology
protein function
Immunohistochemistry
female
spontaneous abortion
medicine.anatomical_structure
priority journal
embryonic structures
protein degradation
proteinase
Healthy Living
decidua
medicine.medical_specialty
Proteases
matrix metalloproteinase
CD56 antigen
first trimester pregnancy
Antigens, Differentiation, Myelomonocytic
Antigens, CD56
macrophage
pregnancy termination
Biology
Protein degradation
Natural killer cell
reverse transcription polymerase chain reaction
Andrology
Antigens, CD
Internal medicine
urokinase receptor
Matrix Metalloproteinase 14
Genetics
medicine
Humans
controlled study
human
protein expression
Molecular Biology
UNK cells
human cell
Cell Biology
natural killer cell
human tissue
Urokinase receptor
Pregnancy Trimester, First
Matrix metalloproteinases
Matrix Metalloproteinase 20
Endocrinology
Reproductive Medicine
protein analysis
gene expression
Healthy for Life
Peptide Hydrolases
Developmental Biology
Subjects
Details
- ISSN :
- 14602407 and 13609947
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- Molecular Human Reproduction
- Accession number :
- edsair.doi.dedup.....115c14f12fce8fc6f90609c937adbad2