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Nucleolin interacts with influenza A nucleoprotein and contributes to viral ribonucleoprotein complexes nuclear trafficking and efficient influenza viral replication
- Source :
- Scientific Reports, Scientific Reports, Nature Publishing Group, 2016, 6, pp.29006. ⟨10.1038/srep29006⟩, Scientific Reports, 2016, 6, pp.29006. ⟨10.1038/srep29006⟩
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Influenza viruses replicate their single-stranded RNA genomes in the nucleus of infected cells and these replicated genomes (vRNPs) are then exported from the nucleus to the cytoplasm and plasma membrane before budding. To achieve this export, influenza viruses hijack the host cell export machinery. However, the complete mechanisms underlying this hijacking remain not fully understood. We have previously shown that influenza viruses induce a marked alteration of the nucleus during the time-course of infection and notably in the nucleolar compartment. In this study, we discovered that a major nucleolar component, called nucleolin, is required for an efficient export of vRNPs and viral replication. We have notably shown that nucleolin interacts with the viral nucleoprotein (NP) that mainly constitutes vRNPs. Our results suggest that this interaction could allow vRNPs to “catch” the host cell export machinery, a necessary step for viral replication.
- Subjects :
- 0301 basic medicine
viruses
Active Transport, Cell Nucleus
RNA-binding protein
Biology
medicine.disease_cause
Virus Replication
Article
Madin Darby Canine Kidney Cells
03 medical and health sciences
Dogs
Influenza A virus
medicine
Influenza A Virus
Animals
Humans
Ribonucleoprotein
Cell Nucleus
Multidisciplinary
Influenza A Virus, H3N2 Subtype
Viral Core Proteins
RNA
RNA-Binding Proteins
Nucleocapsid Proteins
Phosphoproteins
Virology
[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology
Active Transport
3. Good health
Nucleoprotein
Cell nucleus
030104 developmental biology
medicine.anatomical_structure
Viral replication
A549 Cells
H3N2 Subtype
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
[SDV.IMM]Life Sciences [q-bio]/Immunology
Nucleolin
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 6
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....11473f57fe2a3b969f35e5ca5b2bcc50
- Full Text :
- https://doi.org/10.1038/srep29006