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Disruption of the Lotus japonicus transporter LjNPF2.9 increases shoot biomass and nitrate content without affecting symbiotic performances
- Source :
- BMC Plant Biology, BMC Plant Biology, Vol 19, Iss 1, Pp 1-14 (2019), BMC Plant Biology, BioMed Central, 2019, 19 (1), ⟨10.1186/s12870-019-1978-5⟩, BMC Plant Biology 1 (19), . (2019), BMC plant biology (Online) 19 (2019). doi:10.1186/s12870-019-1978-5, info:cnr-pdr/source/autori:Sol S, Valkov TV, Rogato A, Noguero M, Gargiulo L, Mele G, Lacombe B, Chiurazzi M/titolo:Disruption of the Lotus japonicus transporter LjNPF2.9 increases shoot biomass and nitrate content without affecting symbiotic performances/doi:10.1186%2Fs12870-019-1978-5/rivista:BMC plant biology (Online)/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume:19
- Publication Year :
- 2019
-
Abstract
- Background After uptake from soil into the root tissue, distribution and allocation of nitrate throughout the whole plant body, is a critical step of nitrogen use efficiency (NUE) and for modulation of plant growth in response to various environmental conditions. In legume plants nitrate distribution is also important for the regulation of the nodulation process that allows to fix atmospheric N (N2) through the symbiotic interaction with rhizobia (symbiotic nitrogen fixation, SNF). Results Here we report the functional characterization of the Lotus japonicus gene LjNPF2.9, which is expressed mainly in the root vascular structures, a key localization for the control of nitrate allocation throughout the plant body. LjNPF2.9 expression in Xenopus laevis oocytes induces 15NO3 accumulation indicating that it functions as a nitrate importer. The phenotypic characterization of three independent knock out mutants indicates an increased shoot biomass in the mutant backgrounds. This phenotype is associated to an increased/decreased nitrate content detected in the shoots/roots. Furthermore, our analysis indicates that the accumulation of nitrate in the shoot does not affect the nodulation and N-Fixation capacities of the knock out mutants. Conclusions This study shows that LjNPF2.9 plays a crucial role in the downward transport of nitrate to roots, occurring likely through a xylem-to-phloem loading-mediated activity. The increase of the shoot biomass and nitrate accumulation might represent a relevant phenotype in the perspective of an improved NUE and this is further reinforced in legume plants by the reported lack of effects on the SNF efficiency. Electronic supplementary material The online version of this article (10.1186/s12870-019-1978-5) contains supplementary material, which is available to authorized users.
- Subjects :
- transport de nitrate
0106 biological sciences
0301 basic medicine
nitrogen nutrition
Plant Science
01 natural sciences
Plant Roots
croissance et développement végétal
chemistry.chemical_compound
Nitrate
Nitrate distribution
xenopus oocyte
lcsh:Botany
Biomass
condition environnementale
Legume
Plant Proteins
2. Zero hunger
Vegetal Biology
lotus japonicus
food and beverages
Legumes
nutrition azotée
lcsh:QK1-989
Nitrate transport
Shoot
Nitrogen fixation
Plant Shoots
Research Article
Lotus japonicus
Biology
Rhizobia
03 medical and health sciences
Botany
[SDV.BV]Life Sciences [q-bio]/Vegetal Biology
Insertion mutants
Nitrogen use efficiency
Symbiosis
Nitrates
fungi
Membrane Transport Proteins
15. Life on land
biology.organism_classification
030104 developmental biology
chemistry
environmental condition
Lotus
Plant nutrition
Biologie végétale
010606 plant biology & botany
Subjects
Details
- ISSN :
- 14712229
- Volume :
- 19
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- BMC plant biology
- Accession number :
- edsair.doi.dedup.....11394b2247bc4642958e8e2603e7bd30