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Establishment of a clinically relevant large animal model to assess the healing of metaphyseal bone
- Source :
- European Cells & Materials, Vol 37, Pp 444-466 (2019)
- Publication Year :
- 2019
- Publisher :
- AO Research Institute Davos, 2019.
-
Abstract
- Despite the high incidence of metaphyseal bone fractures in patients, the mechanisms underlying the healing processes are poorly understood due to the lack of suitable experimental animal models. Hence, the present study was conducted to establish and characterise a clinically relevant large-animal model for metaphyseal bone healing. Six female adult Merino sheep underwent full wedge-shaped osteotomy at the distal left femur metaphysis. The osteotomy was stabilised internally with a customised anatomical locking titanium plate that allowed immediate post-operative full-weight bearing. Bone healing was evaluated at 12 weeks post-fracture relative to the untouched right femur. Histological and quantitative micro-computed tomography results revealed an increased mineralised bone mass with a rich bone microarchitecture. New trabeculae healed by direct intramembranous ossification, without callus and cartilaginous tissue formation. Stiffness at the cortical and trabecular regions was comparable in both groups. Functional morphological analysis of the osteocyte lacunae revealed regularly arranged spherically shaped lacunae along with the canalicular network. Bone surface biochemical analysis using time-of-flight secondary-ion mass spectrometry showed high and homogeneously distributed levels of calcium and collagenous components. Ultrastructure imaging of the new trabeculae revealed a characteristic parallel arrangement of the collagen fibrils, evenly mineralised by the dense mineral substance. The specialised bone cells were also characterised by their unique structural features. Bone remodelling in the fractured femur was evident in the higher expression levels of prominent bone formation and resorption genes. In conclusion, the novel metaphyseal fracture model is beneficial for studying healing and treatment options for the enhancement of metaphyseal bone defects.
- Subjects :
- lcsh:Diseases of the musculoskeletal system
medicine.medical_treatment
microstructure
lcsh:Surgery
Bone healing
Metaphysis
adult sheep
Osteotomy
biomechanics
Bone remodeling
Osteogenesis
Bone cell
medicine
Animals
Femur
Bony Callus
metaphysis
Fracture Healing
Sheep
business.industry
Anatomy
lcsh:RD1-811
ultrastructure
Disease Models, Animal
medicine.anatomical_structure
Osteocyte
Intramembranous ossification
Calcium
Female
lcsh:RC925-935
business
Femoral Fractures
locking plate
Subjects
Details
- Language :
- English
- ISSN :
- 14732262
- Volume :
- 37
- Database :
- OpenAIRE
- Journal :
- European Cells & Materials
- Accession number :
- edsair.doi.dedup.....1138cdbd94f10402449e988b5b3a7d69