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Effects of kisspeptin1 on electrical activity of an extrahypothalamic population of gonadotropin-releasing hormone neurons in medaka (Oryzias latipes)
- Source :
- PLoS ONE, PloS one, vol 7, iss 5, PLoS ONE, Vol 7, Iss 5, p e37909 (2012), Zhao, Y; & Wayne, NL. (2012). Effects of Kisspeptin1 on electrical activity of an extrahypothalamic population of gonadotropin-releasing hormone neurons in medaka (Oryzias latipes). PLoS ONE, 7(5). doi: 10.1371/journal.pone.0037909. UCLA: Retrieved from: http://www.escholarship.org/uc/item/7mx9700n
- Publication Year :
- 2011
-
Abstract
- Kisspeptin (product of the kiss1 gene) is the most potent known activator of the hypothalamo-pituitary-gonadal axis. Both kiss1 and the kisspeptin receptor are highly expressed in the hypothalamus of vertebrates, and low doses of kisspeptin have a robust and long-lasting stimulatory effect on the rate of action potential firing of hypophysiotropic gonadotropin releasing hormone-1 (GnRH1) neurons in mice. Fish have multiple populations of GnRH neurons distinguished by their location in the brain and the GnRH gene that they express. GnRH3 neurons located in the terminal nerve (TN) associated with the olfactory bulb are neuromodulatory and do not play a direct role in regulating pituitary-gonadal function. In medaka fish, the electrical activity of TN-GnRH3 neurons is modulated by visual cues from conspecifics, and is thought to act as a transmitter of information from the external environment to the central nervous system. TN-GnRH3 neurons also play a role in sexual motivation and arousal states, making them an important population of neurons to study for understanding coordination of complex behaviors. We investigated the role of kisspeptin in regulating electrical activity of TN-GnRH3 neurons in adult medaka. Using electrophysiology in an intact brain preparation, we show that a relatively brief treatment with 100 nM of kisspeptin had a long-lasting stimulatory effect on the electrical activity of an extrahypothalamic population of GnRH neurons. Dose-response analysis suggests a relatively narrow activational range of this neuropeptide. Further, blocking action potential firing with tetrodotoxin and blocking synaptic transmission with a low Ca2+/high Mg2+solution inhibited the stimulatory action of kisspeptin on electrical activity, indicating that kisspeptin is acting indirectly through synaptic regulation to excite TN-GnRH3 neurons. Our findings provide a new perspective on kisspeptin's broader functions within the central nervous system, through its regulation of an extrahypothalamic population of GnRH neurons involved in multiple neuromodulatory functions. © 2012 Zhao, Wayne.
- Subjects :
- Male
Central Nervous System
Patch-Clamp Techniques
Oryzias
lcsh:Medicine
Action Potentials
Gonadotropin-releasing hormone
Synaptic Transmission
Gonadotropin-Releasing Hormone
0302 clinical medicine
Kisspeptin
Molecular Cell Biology
lcsh:Science
Neurons
0303 health sciences
education.field_of_study
Kisspeptins
Multidisciplinary
Neuromodulation
Neurochemistry
Animal Models
Olfactory Bulb
Hypothalamus
Homeostatic Mechanisms
Neurological
Female
Drug
Cellular Types
hormones, hormone substitutes, and hormone antagonists
Ichthyology
Research Article
medicine.medical_specialty
Neural Networks
General Science & Technology
1.1 Normal biological development and functioning
Population
Molecular Sequence Data
Neuropeptide
Neurophysiology
030209 endocrinology & metabolism
Biology
Neurotransmission
Dose-Response Relationship
03 medical and health sciences
Model Organisms
Species Specificity
Cellular neuroscience
Underpinning research
Internal medicine
medicine
Animals
Amino Acid Sequence
education
030304 developmental biology
Analysis of Variance
Dose-Response Relationship, Drug
Contraception/Reproduction
lcsh:R
Neurosciences
Neuroendocrinology
Electrophysiology
Endocrinology
nervous system
Cellular Neuroscience
lcsh:Q
Neuroscience
Zoology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- PloS one
- Accession number :
- edsair.doi.dedup.....1131ee12fa4416f84471a3bdd695d55d
- Full Text :
- https://doi.org/10.1371/journal.pone.0037909.