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Melimine-Coated Antimicrobial Contact Lenses Reduce Microbial Keratitis in an Animal Model
- Source :
- Investigative ophthalmologyvisual science. 57(13)
- Publication Year :
- 2016
-
Abstract
- PURPOSE. To determine the ability of antimicrobial peptide melimine-coated contact lenses to reduce the incidence of microbial keratitis (MK) in a rabbit model of contact lens wear. METHODS. In vitro antimicrobial activity of melimine-coated contact lenses was determined against Pseudomonas aeruginosa by viable count and a radiolabeled assay. The amount of lipopolysaccharide (LPS) associated with bacteria bound to melimine-coated and control lenses was determined. Ocular swabs from rabbit eyes were collected for assessment of ocular microflora. A rabbit model for MK was developed that used overnight wear of contact lenses colonized by P. aeruginosa in the absence of a corneal scratch. During lens wear, detailed ocular examinations were performed, and the incidence of MK was investigated. Bacteria associated with worn lenses and infected corneas were determined by viable plate count. RESULTS. Inhibition in viable and total P. aeruginosa adhesion by melimine-coated contact lenses was 3.1 log10 and 0.4 log10, respectively. After colonization, the amount of LPS on lenses was approximately the same with or without melimine. Gram-positive bacteria were found in all the ocular swabs followed by fungus (42%). Melimine-coated lens wear was protective and significantly (odds ratio 10.12; P = 0.012) reduced the incidence of P. aeruginosa-driven MK in the rabbit model. The antimicrobial lenses were associated with significantly (P < 0.001) lower ocular scores, indicating improved ocular signs compared with controls. CONCLUSIONS. This study showed that contaminated contact lenses can produce MK without corneal epithelial defect in an animal model. Melimine-coated contact lenses reduced the incidence of MK associated with P. aeruginosa in vivo. Development of MK requires viable bacteria adherent to contact lenses, and bacterial debris adherent at the lens surface did not cause keratitis.
- Subjects :
- 0301 basic medicine
genetic structures
Contact Lenses
Colony Count, Microbial
medicine.disease_cause
Bacterial Adhesion
Eye Infections, Bacterial
Keratitis
law.invention
Microbiology
Cornea
03 medical and health sciences
0302 clinical medicine
Anti-Infective Agents
Coated Materials, Biocompatible
In vivo
law
medicine
Animals
Prospective Studies
Chemistry
Pseudomonas aeruginosa
Eye infection
medicine.disease
Antimicrobial
eye diseases
Contact lens
Lens (optics)
Disease Models, Animal
030104 developmental biology
Viable count
030221 ophthalmology & optometry
sense organs
Rabbits
Antimicrobial Cationic Peptides
Follow-Up Studies
Subjects
Details
- ISSN :
- 15525783 and 01460404
- Volume :
- 57
- Issue :
- 13
- Database :
- OpenAIRE
- Journal :
- Investigative ophthalmologyvisual science
- Accession number :
- edsair.doi.dedup.....113018039f1c5e50ebf04436ab27058b