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The gene therapy of collagen-induced arthritis in rats by intramuscular administration of the plasmid encoding TNF-binding domain of variola virus CrmB protein
- Source :
- Doklady. Biochemistry and biophysics. 469(1)
- Publication Year :
- 2016
-
Abstract
- Wistar rats with collagen-induced arthritis were intramuscularly injected with the recombinant plasmid pcDNA/sTNF-BD encoding the sequence of the TNF-binding protein domain of variola virus CrmB protein (VARV sTNF-BD) or the pcDNA3.1 vector. Quantitative analysis showed that the histopathological changes in the hind-limb joints of rats were most severe in the animals injected with pcDNA3.1 and much less severe in the group of rats injected with pcDNA/sTNF-BD, which indicates that gene therapy of rheumatoid arthritis is promising in the case of local administration of plasmids governing the synthesis of VARV immunomodulatory proteins.
- Subjects :
- 0301 basic medicine
Male
Genetic enhancement
Protein domain
Genetic Vectors
Biophysics
Arthritis
Biology
Biochemistry
Injections, Intramuscular
law.invention
Arthritis, Rheumatoid
03 medical and health sciences
Viral Proteins
Plasmid
law
medicine
Animals
Rats, Wistar
Synovitis
General Chemistry
General Medicine
Genetic Therapy
Variola virus
medicine.disease
Molecular biology
Arthritis, Experimental
Hindlimb
030104 developmental biology
Treatment Outcome
Recombinant DNA
Tumor necrosis factor alpha
Female
Carrier Proteins
Binding domain
Subjects
Details
- ISSN :
- 16083091
- Volume :
- 469
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Doklady. Biochemistry and biophysics
- Accession number :
- edsair.doi.dedup.....112f0411b6b484387b10afa7b1577758