Back to Search
Start Over
Sublethal doses of neonicotinoid imidacloprid can interact with honey bee chemosensory protein 1 (CSP1) and inhibit its function
- Source :
- Biochemical and biophysical research communications. 486(2)
- Publication Year :
- 2017
-
Abstract
- As a frequently used neonicotinoid insecticide, imidacloprid can impair the chemoreceptive behavior of honey bees even at sublethal doses, while the physiochemical mechanism has not been further revealed. Here, multiple fluorescence spectra, thermodynamic method, and molecular docking were used to study the interaction and the functional inhibition of imidacloprid to the recombinant CSP1 protein in Asian honey bee, Apis cerana. The results showed that the fluorescence intensity (λem = 332 nm) of CSP1 could be significantly quenched by imidacloprid in a dynamic mode. During the quenching process, ΔH > 0, ΔS > 0, indicating that the acting forces of imidacloprid with CSP1 are mainly hydrophobic interactions. Synchronous fluorescence showed that the fluorescence of CSP1 was mainly derived from tryptophan, and the hydrophobicity of tryptophan decreased with the increase of imidacloprid concentration. Molecular docking predicted the optimal pose and the amino acid composition of the binding process. Circular dichroism (CD) spectra showed that imidacloprid reduced the α-helix of CSP1 and caused the extension of the CSP1 peptide chain. In addition, the binding of CSP1 to floral scent β-ionone was inhibited by nearly 50% of the apparent association constant (KA) in the presence of 0.28–2.53 ng/bee of imidacloprid, and the inhibition rate of nearly 95% at 3.75 ng/bee of imidacloprid at sublethal dose level. This study initially revealed the molecular physiochemical mechanism that sublethal doses of neonicotinoid still interact and inhibit the physiological function of the honey bees' chemoreceptive system.
- Subjects :
- 0301 basic medicine
Circular dichroism
Insecticides
Biophysics
Cholinergic Agents
Gene Expression
Peptide
Biology
Biochemistry
Protein Structure, Secondary
03 medical and health sciences
chemistry.chemical_compound
Neonicotinoids
Protein Domains
Imidacloprid
parasitic diseases
Botany
Escherichia coli
Animals
Amino Acid Sequence
Cloning, Molecular
Molecular Biology
Apis cerana
chemistry.chemical_classification
fungi
Neonicotinoid
Tryptophan
Imidazoles
Chemosensory protein
Cell Biology
Honey bee
Feeding Behavior
Bees
biology.organism_classification
Nitro Compounds
Recombinant Proteins
Molecular Docking Simulation
Kinetics
030104 developmental biology
Spectrometry, Fluorescence
chemistry
Insect Proteins
Thermodynamics
Tyrosine
Norisoprenoids
Sequence Alignment
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 486
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....112217dad1cdafdb42cbfdf48ac98d1a