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Characteristics of the transport of carnitine into the cauda epididymidis of the rat as ascertained by luminal perfusion in vitro
- Source :
- International journal of andrology. 9(5)
- Publication Year :
- 1986
-
Abstract
- An in vitro luminal perfusion technique was used to monitor the transepithelial movement of [3H]L-carnitine into the cauda epididymal lumen of rats. Movement of the volume marker [14C]inulin into the lumen was limited while the transport of [3H]L-carnitine was saturable and was dependent on respiratory energy. The transport of L-carnitine (50 microM) was significantly inhibited by 500 microM D-carnitine, palmitoyl L-carnitine and deoxycarnitine but not by trimethylammonium iodide, trimethyl phosphate, acetyl-L-carnitine, choline, betaine, gamma-aminobutyric acid or DL-carnitinamide. The Kt (116 microM) and Vmax (0.1 nmol/30 min/cm) for the carriers were calculated from the present data and as the Kt is about twice the normal plasma concentration of carnitine, the rate of transport by the pump is presumed to be limited by the plasma concentration.
- Subjects :
- Male
medicine.medical_specialty
Urology
Endocrinology, Diabetes and Metabolism
Lumen (anatomy)
Biological Transport, Active
Biology
In Vitro Techniques
Carnitine transport
chemistry.chemical_compound
Betaine
Internal medicine
Carnitine
medicine
Choline
Animals
Respiratory system
Epididymis
Inulin
Rats, Inbred Strains
Stereoisomerism
Metabolism
Rats
Perfusion
Kinetics
Endocrinology
Reproductive Medicine
chemistry
Chromatography, Thin Layer
medicine.drug
Subjects
Details
- ISSN :
- 01056263
- Volume :
- 9
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- International journal of andrology
- Accession number :
- edsair.doi.dedup.....11117aaa78f6086a6b075494148e7584