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Dopaminergic regulation of BDNF content in the pituitary intermediate lobe
- Source :
- NeuroReport. 8:1089-1093
- Publication Year :
- 1997
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1997.
-
Abstract
- Previous studies in adult rats have demonstrated that the neurotrophin, brain-derived neurotrophic factor (BDNF), is present in virtually all cells of the pituitary intermediate lobe. In the present study, we demonstrate that cells cultured from adult intermediate lobe pituitary (ILP) rapidly lose their BDNF immunoreactivity (IR). Furthermore, a similar loss of immunostaining occurs in whole (undissociated) ILP within 30 min after removal from the rat. However, when the dopamine agonist apomorphine is present throughout the dissociation procedure and during cultivation, BDNF-IR is preserved. Supplying apomorphine only during either dissociation or cultivation did not prevent the loss of BDNF-IR in the 24 h cultures. These results suggest that a tonic dopaminergic stimulus is required to maintain BDNF-IR in ILP cells.
- Subjects :
- Male
Pituitary gland
medicine.medical_specialty
Apomorphine
Dopamine
Biology
Rats, Sprague-Dawley
chemistry.chemical_compound
Neurotrophic factors
Internal medicine
medicine
Animals
Neurotransmitter
Cells, Cultured
Brain-derived neurotrophic factor
Brain-Derived Neurotrophic Factor
General Neuroscience
Dopaminergic
Immunohistochemistry
Rats
medicine.anatomical_structure
Endocrinology
chemistry
Pituitary Gland
Dopamine Agonists
biology.protein
Female
Endocrine gland
medicine.drug
Neurotrophin
Subjects
Details
- ISSN :
- 09594965
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- NeuroReport
- Accession number :
- edsair.doi.dedup.....11114a7518493f0fe7f3625b5c115c79