Back to Search
Start Over
Microbial Epoxidation of cis -Propenylphosphonic to (−)- cis -1,2-Epoxypropylphosphonic Acid
- Source :
- Applied Microbiology. 22:55-60
- Publication Year :
- 1971
- Publisher :
- American Society for Microbiology, 1971.
-
Abstract
- Eighteen species of Penicillium , one of Oidium and one of Paecilomyces were found to effect a stereospecific conversion of cis -propenylphosphonate to fosfomycin which was identified by paper chromatography and gas-liquid chromatography (GLC) of the trimethylsilyl esters. Penicillium spinulosum carried out the epoxidation only after the glucose substrate had been utilized. Glucose controlled the epoxidation since its residual concentrations in the broth severely depresses the reaction. At optimum levels of glucose, an epoxidation efficiency approaching 90% of olefin charged (0.2 g/liter) was obtained after 10 days of incubation. The olefin concentration could be increased to 0.5 g/liter when glucose was replaced by glycerol, whereby a 90% conversion to fosfomycin was attainable in 6 days. The high conversion efficiency, a good agreement between the GLC assay and bioactivity, are indicative of the levorotatory nature of the product.
- Subjects :
- Glycerol
Chromatography, Gas
Chemical Phenomena
Trimethylsilyl
Chromatography, Paper
Organophosphonates
Alkenes
General Biochemistry, Genetics and Molecular Biology
chemistry.chemical_compound
Ethers, Cyclic
General Pharmacology, Toxicology and Pharmaceutics
Metabolism and Products
Olefin fiber
Chromatography
General Immunology and Microbiology
Penicillium
Substrate (chemistry)
Stereoisomerism
General Medicine
Proteus
Anti-Bacterial Agents
Culture Media
Chemistry
Paper chromatography
Glucose
chemistry
Fermentation
Carbohydrate Metabolism
Biological Assay
Mitosporic Fungi
Gas chromatography
Penicillium spinulosum
Subjects
Details
- ISSN :
- 00036919
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Applied Microbiology
- Accession number :
- edsair.doi.dedup.....110df93e494a4061a2f90a8b078487ed