Back to Search
Start Over
Anticancer effects of deproteinized asparagus polysaccharide on hepatocellular carcinoma in vitro and in vivo
- Source :
- Tumor Biology. 35:3517-3524
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- Hepatocellular carcinoma (HCC) is one of the most aggressive malignancies in the world whose chemoprevention became increasingly important in HCC treatment. Although the anticancer effects of asparagus constituents have been investigated in several cancers, its effects on hepatocellular carcinoma have not been fully studied. In this study, we investigated the anticancer effects of the deproteinized asparagus polysaccharide on the hepatocellular carcinoma cells using the in vitro and in vivo experimental model. Our data showed that deproteinized asparagus polysaccharide might act as an effective inhibitor on cell growth in vitro and in vivo and exert potent selective cytotoxicity against human hepatocellular carcinoma Hep3B and HepG2 cells. Further study showed that it could potently induce cell apoptosis and G2/M cell cycle arrest in the more sensitive Hep3B and HepG2 cell lines. Moreover, deproteinized asparagus polysaccharide potentiated the effects of mitomycin both in vitro and in vivo. Mechanistic studies revealed that deproteinized asparagus polysaccharide might exert its activity through an apoptosis-associated pathway by modulating the expression of Bax, Bcl-2, and caspase-3. In conclusion, deproteinized asparagus polysaccharide exhibited significant anticancer activity against hepatocellular carcinoma cells and could sensitize the tumoricidal effects of mitomycin, indicating that it is a potential therapeutic agent (or chemosensitizer) for liver cancer therapy.
- Subjects :
- Carcinoma, Hepatocellular
Mitomycin
Chemosensitizer
Apoptosis
Polysaccharides
In vivo
Cell Line, Tumor
medicine
Humans
Asparagus
Cell Proliferation
biology
Caspase 3
Cell growth
Liver Neoplasms
food and beverages
Cell Cycle Checkpoints
General Medicine
medicine.disease
biology.organism_classification
Antineoplastic Agents, Phytogenic
digestive system diseases
In vitro
Biochemistry
Cell culture
Hepatocellular carcinoma
Cancer research
Asparagus Plant
Subjects
Details
- ISSN :
- 14230380 and 10104283
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Tumor Biology
- Accession number :
- edsair.doi.dedup.....110664a6cd8573f25ceb51ea82d96200