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Trans-portal hepatic infusion of cultured bone marrow-derived mesenchymal stem cells in a steatohepatitis murine model
- Source :
- Journal of Clinical Biochemistry and Nutrition
- Publication Year :
- 2020
- Publisher :
- the Society for Free Radical Research Japan, 2020.
-
Abstract
- The incidence of nonalcoholic steatohepatitis-related liver cirrhosis is increasing. We used a steatohepatitis murine model fed a choline-deficient, l-amino acid-defined (CDAA) diet with a single injection of carbon tetrachloride (CCl4) to evaluate the efficacy of trans-portal hepatic infusion of bone marrow-derived mesenchymal stem cells (BMSCs) for liver fibrosis, liver steatosis, and oxidative stress. Mice were fed a CDAA diet and injected with a single intraperitoneal dose of CCl4 (0.5 ml/kg) after 4 weeks of CDAA diet. After 12 weeks of CDAA diet, 1 × 106 luciferase-positive syngeneic BMSCs (Luc-BMSCs) were infused into the animal spleen. An in vivo imaging system was used to confirm Luc-BMSC accumulation in the liver via the portal vein, and at 4 weeks after infusion, we compared liver fibrosis, liver steatosis, and oxidative stress. After the BMSC-infusion, serum albumin and serum total bilirubin were significantly improved. Liver fibrosis assessed by Sirius red staining, α-smooth muscle actin protein, and collagen 1A1 mRNA expression was significantly suppressed. Furthermore, liver steatosis area was significantly lower, the 8-hydroxy-2'-deoxyguanosine-positive cells were significantly fewer, and superoxide dismutase 2 protein expression of the liver was significantly increased. In conclusion, our data confirmed the efficacy of trans-portal hepatic infusion of BMSCs in a steatohepatitis murine model.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
Cirrhosis
macrophage polarization
Clinical Biochemistry
Serum albumin
Medicine (miscellaneous)
CCL4
Spleen
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Internal medicine
medicine
oxidative stress
nonalcoholic steatohepatitis
Sirius Red
mesenchymal stem cell
liver fibrosis
030109 nutrition & dietetics
Nutrition and Dietetics
biology
business.industry
medicine.disease
medicine.anatomical_structure
Endocrinology
chemistry
Carbon tetrachloride
biology.protein
030211 gastroenterology & hepatology
Original Article
Bone marrow
Steatohepatitis
business
Subjects
Details
- Language :
- English
- ISSN :
- 18805086 and 09120009
- Volume :
- 67
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of Clinical Biochemistry and Nutrition
- Accession number :
- edsair.doi.dedup.....10fb87e0629b2ce09f7e13dade7ae1e8