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TRIB3 promotes proliferation, migration, and invasion of retinoblastoma cells by activating the AKT/mTOR signaling pathway
- Source :
- Cancer biomarkers : section A of Disease markers. 31(4)
- Publication Year :
- 2021
-
Abstract
- BACKGROUND: Tribbles pseudokinase 3 (TRIB3) is a member of the tribbles-related family, which has been determined in various cancers, including renal cell carcinoma, acute promyelocytic leukemia, colorectal cancer, endometrial cancer, and glioma. However, its role in retinoblastoma (RB) has not yet been explored. METHODS: The expression level of TRIB3 was detected in RB tissues and cell lines using qRT-PCR. The effects of TRIB3 on cell proliferation and invasion capacities were analyzed with MTT, crystal violet, and transwell assays. Western blot and rescue assays were conducted to explore the underlying mechanism. RESULTS: This study found that TRIB3 was upregulated in human RB tissues compared to adjacent normal tissues both at the mRNA and protein levels. Overexpression of TRIB3 significantly promoted cell proliferation and invasion of RB cells, while TRIB3 knockdown inhibited these processes. Moreover, the mechanism deciphering experiments showed that TRIB3 overexpression can increase AKT and mTOR phosphorylation. Conversely, TRIB3 knockdown decreased the phosphorylation of AKT and mTOR. Additionally, MK2206, a potent AKT inhibitor, blocked the promotive effects of TRIB3 in RB cells. CONCLUSION: This study demonstrated that TRIB3 acts as an oncogene and plays a crucial role in the proliferation and invasion of RB cells via regulating the AKT/mTOR signaling pathway. Therefore, TRIB3 may serve as a potential target in the diagnosis and/or treatment of RB.
- Subjects :
- Male
Cancer Research
Cell Cycle Proteins
Protein Serine-Threonine Kinases
03 medical and health sciences
0302 clinical medicine
Cell Movement
Genetics
medicine
Humans
Neoplasm Invasiveness
Protein kinase B
PI3K/AKT/mTOR pathway
030304 developmental biology
Cell Proliferation
0303 health sciences
Gene knockdown
Oncogene
Cell growth
Chemistry
Retinoblastoma
TOR Serine-Threonine Kinases
General Medicine
medicine.disease
Repressor Proteins
Oncology
TRIB3
030220 oncology & carcinogenesis
Child, Preschool
Cancer research
Phosphorylation
Female
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 18758592
- Volume :
- 31
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Cancer biomarkers : section A of Disease markers
- Accession number :
- edsair.doi.dedup.....10f9766fd537c01abe3e67b5355f2efc