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An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex
- Source :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET, Journal of experimental botany 67 (2016): 1965–1978. doi:10.1093/jxb/erw018, info:cnr-pdr/source/autori:Siena L.A.; Ortiz J.P.A.; Calderini O.; Paolocci F.; Caceres M.E.; Kaushal P.; Grisan S.; Pessino S.C.; Pupilli F./titolo:An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex/doi:10.1093%2Fjxb%2Ferw018/rivista:Journal of experimental botany/anno:2016/pagina_da:1965/pagina_a:1978/intervallo_pagine:1965–1978/volume:67
- Publication Year :
- 2016
- Publisher :
- Oxford University Press, 2016.
-
Abstract
- Apomixis in plants consists of asexual reproduction by seeds. Here we characterized at structural and functional levels an apomixis-linked sequence of Paspalum simplex homologous to subunit 3 of the ORIGIN RECOGNITION COMPLEX (ORC3). ORC is a multiprotein complex which controls DNA replication and cell differentiation in eukaryotes. Three PsORC3 copies were identified, each one characterized by a specific expression profile. Of these, PsORC3a, specific for apomictic genotypes, is a pseudogene that was poorly and constitutively expressed in all developmental stages of apomictic flowers, whereas PsORC3b, the putative functional gene in sexual flowers, showed a precise time-related regulation. Sense transcripts of PsORC3 were expressed in the female cell lineage of both apomictic and sexual reproductive phenotypes, and in aposporous initials. Although strong expression was detected in sexual early endosperm, no expression was present in the apomictic endosperm. Antisense PsORC3 transcripts were revealed exclusively in apomictic germ cell lineages. Defective orc3 mutants of rice and Arabidopsis showed normal female gametophytes although the embryo and endosperm were arrested at early phases of development. We hypothesize that PsORC3a is associated with the down-regulation of its functional homolog and with the development of apomictic endosperm which deviates from the canonical 2(maternal):1(paternal) genome ratio. Fil: Siena, Lorena Adelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina Fil: Calderini, Ornella. Istituto di Bioscienze e Biorisorse; Italia Fil: Paolocci, Francesco. Istituto di Bioscienze e Biorisorse; Italia Fil: Cáceres, Maria E.. Istituto di Bioscienze e Biorisorse; Italia Fil: Kaushal, Pankaj. Istituto di Bioscienze e Biorisorse; Italia Fil: Grisan, Simone. Istituto di Bioscienze e Biorisorse; Italia Fil: Pessino, Silvina Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Investigaciones en Ciencias Agrarias de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Instituto de Investigaciones en Ciencias Agrarias de Rosario; Argentina Fil: Pupilli, Fulvio. Istituto di Bioscienze e Biorisorse; Italia
- Subjects :
- 0106 biological sciences
0301 basic medicine
Transcription, Genetic
Physiology
Cellular differentiation
Arabidopsis
Origin Recognition Complex
Asexual reproduction
Plant Science
Seed Development
01 natural sciences
Endosperm
gene silencing
Gene Expression Regulation, Plant
Paspalum
Cloning, Molecular
In Situ Hybridization
Plant Proteins
Genetics
Gametophyte
Reproduction
Agricultura
Chromosome Mapping
food and beverages
medicine.anatomical_structure
Apomixis
Pseudogenes
Germ cell
seed development
Pseudogene
Biology
03 medical and health sciences
Sequence Homology, Nucleic Acid
medicine
RNA, Messenger
Gene Silencing
Base Sequence
Gene Expression Profiling
Oryza
030104 developmental biology
CIENCIAS AGRÍCOLAS
Mutation
purl.org/becyt/ford/4.1 [https]
Origin recognition complex
origin recognition complex
Agricultura, Silvicultura y Pesca
Sequence Alignment
purl.org/becyt/ford/4 [https]
010606 plant biology & botany
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- CONICET Digital (CONICET), Consejo Nacional de Investigaciones Científicas y Técnicas, instacron:CONICET, Journal of experimental botany 67 (2016): 1965–1978. doi:10.1093/jxb/erw018, info:cnr-pdr/source/autori:Siena L.A.; Ortiz J.P.A.; Calderini O.; Paolocci F.; Caceres M.E.; Kaushal P.; Grisan S.; Pessino S.C.; Pupilli F./titolo:An apomixis-linked ORC3-like pseudogene is associated with silencing of its functional homolog in apomictic Paspalum simplex/doi:10.1093%2Fjxb%2Ferw018/rivista:Journal of experimental botany/anno:2016/pagina_da:1965/pagina_a:1978/intervallo_pagine:1965–1978/volume:67
- Accession number :
- edsair.doi.dedup.....10f82e0789de7d56c5cb2820b7e28bc5