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miR-203 promotes proliferation, migration and invasion by degrading SIK1 in pancreatic cancer
- Source :
- Oncology reports. 35(3)
- Publication Year :
- 2015
-
Abstract
- Pancreatic ductal adenocarcinoma (PDA) is among the most lethal human cancers and it is insensitive to many chemotherapeutic drugs. The molecular basis of pancreatic cancer remains to be elucidated. Investigations into the molecular mechanism involved in the development and progression as well as drug resistance of the disease may be useful to understand the pathogenesis and progression of the disease and offer new targets for effective therapies. In the present study, we showed that salt-inducible kinase 1 (SIK1) was downregulated and loss of SIK1 was associated with gemcitabine resistance in pancreatic cancer. In pancreatic cancer cells, SIK1 inhibited proliferation, migration and invasion. An analysis of potential microRNA target sites was performed using the prediction algorithms, miRanda, TargetScan and PicTar. The three algorithms predicted that miR-203 is capable of targeting 3'UTR of SIK1. Subsequent experiments confirmed the prediction. In addition, the results showed that miR-203 promotes proliferation, migration and invasion in pancreatic cancer cells, whereas the restoration of SIK1 abrogated the regulation of pre-miR‑203-mediated proliferation, migration and invasion.
- Subjects :
- 0301 basic medicine
Cancer Research
Adenocarcinoma
Protein Serine-Threonine Kinases
Bioinformatics
Deoxycytidine
03 medical and health sciences
0302 clinical medicine
Cell Movement
Pancreatic cancer
Cell Line, Tumor
microRNA
Medicine
Humans
Neoplasm Invasiveness
Cell Proliferation
Oncogene
Kinase
business.industry
Cancer
General Medicine
Cell cycle
medicine.disease
Molecular medicine
Gemcitabine
Gene Expression Regulation, Neoplastic
Pancreatic Neoplasms
MicroRNAs
030104 developmental biology
Oncology
Drug Resistance, Neoplasm
030220 oncology & carcinogenesis
Cancer research
business
miR-203
Signal Transduction
Subjects
Details
- ISSN :
- 17912431
- Volume :
- 35
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Oncology reports
- Accession number :
- edsair.doi.dedup.....10e4316ed9e3078780e9d972938d24e7