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Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio)
- Source :
- Aquatic toxicology (Amsterdam, Netherlands).
- Publication Year :
- 2011
-
Abstract
- This study examined the disruptive effect of exposure to polybrominated diphenyl ethers (PBDEs) on retinoid content in zebrafish (Danio rerio). Adult zebrafish were exposed to an environmentally relevant concentration (0.45 μg/L) and a higher concentration (9.6 μg/L) of DE-71 for 60 days. Retinoid content and gene transcription levels were examined in female zebrafish. PBDE exposure caused a significant decrease of retinyl ester content in the intestine and a downregulation of intestinal cellular retinol binding protein gene transcription (CRBP1a). In the liver, retinyl ester content was significantly decreased, while retinol content was increased. An upregulation of liver CRBP2a and retinol binding protein (RBP) gene transcription and an increased level of RBP protein were observed. In the eyes, both the retinal and retinyl ester content were increased and CRBP1a gene transcription was upregulated. However, the gene encoding for retinal dehydrogenase (RALDH2), responsible for retinoic acid synthesis, was downregulated in the eyes. CYP26a, the gene responsible for retinoic acid degradation, was upregulated, which indicated an increased level of retinoic acid. In the ovaries, the increased deposition of retinoids was also observed, while gene transcription levels of both CRBPs (CRBP1a and CRBP1b) were upregulated. An increased deposition of retinal was measured in the eggs. Overall, this study demonstrated that long-term exposure of zebrafish to environmentally relevant concentrations of DE-71 disrupted the transport, storage and metabolism of retinoid in various tissues. This study also indicated that retinoid levels in zebrafish are sensitive to PBDE exposure and highlighted the importance of liver storage, which appears to support important functions in reproduction and vision.
- Subjects :
- Male
medicine.medical_specialty
Retinal dehydrogenase
medicine.drug_class
Health, Toxicology and Mutagenesis
Retinoic acid
Down-Regulation
Aquatic Science
Biology
Real-Time Polymerase Chain Reaction
chemistry.chemical_compound
Random Allocation
Retinoids
Internal medicine
medicine
Halogenated Diphenyl Ethers
Animals
Tissue Distribution
Retinoid
Zebrafish
Chromatography, High Pressure Liquid
Ovum
Regulation of gene expression
Reproduction
Retinol
Retinal Dehydrogenase
Retinal
biology.organism_classification
Retinol-Binding Proteins
Retinol binding protein
Endocrinology
chemistry
Gene Expression Regulation
Female
Environmental Monitoring
Subjects
Details
- ISSN :
- 18791514
- Database :
- OpenAIRE
- Journal :
- Aquatic toxicology (Amsterdam, Netherlands)
- Accession number :
- edsair.doi.dedup.....10d99a43f6655f239fc77284648387e6