Back to Search
Start Over
Chamber-Specific Protein Expression during Direct Cardiac Reprogramming
- Source :
- Cells, Vol 10, Iss 1513, p 1513 (2021), Cells
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Forced expression of core cardiogenic transcription factors can directly reprogram fibroblasts to induced cardiomyocyte-like cells (iCMs) in vitro and in vivo. This cardiac reprogramming approach provides a proof of concept for induced heart regeneration by converting a fibroblast fate to a cardiomyocyte fate. However, it remains elusive whether chamber-specific cardiomyocytes can be generated by cardiac reprogramming. Therefore, we assessed the ability of the cardiac reprogramming approach for chamber specification in vitro and in vivo. We found that in vivo cardiac reprogramming post-myocardial infarction exclusively induces a ventricular-like phenotype, while a major fraction of iCMs generated in vitro failed to determine their chamber identities. Our results suggest that in vivo cardiac reprogramming may have an inherent advantage of generating chamber-matched new cardiomyocytes as a potential heart regenerative approach.
- Subjects :
- 0301 basic medicine
Specific protein
QH301-705.5
Heart Ventricles
Myocardial Infarction
cardiomyocyte
030204 cardiovascular system & hematology
Biology
fibroblast
03 medical and health sciences
Mice
0302 clinical medicine
In vivo
medicine
Animals
Myocytes, Cardiac
Heart Atria
Biology (General)
Fibroblast
Transcription factor
Regeneration (biology)
Communication
reprogramming
chamber
General Medicine
Fibroblasts
Cellular Reprogramming
Phenotype
In vitro
Cell biology
030104 developmental biology
medicine.anatomical_structure
cardiovascular system
Reprogramming
Subjects
Details
- Language :
- English
- ISSN :
- 20734409
- Volume :
- 10
- Issue :
- 1513
- Database :
- OpenAIRE
- Journal :
- Cells
- Accession number :
- edsair.doi.dedup.....10d82f4240b6e72647f5906068ed5b58