Simplified low-pressure high-resolution nucleotide analyser

A simple nucleotide analyser was constructed from commercially available components. A 20 cm × 0.4 cm I.D. column of pellicular anion exchanger operating at approximately 20 p.s.i. was found to perform identically with published methods using high-pressure equipment. The eluant was monitored using a variable-wavelength UV monitor. A very simple device for producing variable gradients was employed. The nueclotide analyser was trouble free to operate and the results were both linear and reproducible. Applications to the rapid analysis of nucleotides and the high-sensitivity analysis (approx. 10 pmoles) of cyclic 3′,5′-adenosine monophosphate and cyclic 2′,3′-guanosine monophosphate and tissue nucleotides are described.