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The effects of ionic liquid 1-ethyl-3-methylimidazolium trifluoromethanesulfonate on the production of 1,3-propanediol from crude glycerol by microbial consortium
- Source :
- Bioprocess and Biosystems Engineering. 41:1079-1088
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- Ionic liquids (ILs) as “green” solvents have been widely used owing to their excellent properties, e.g., for biodiesel production. Crude glycerol as a by-product in biodiesel production is an ideal feedstock for the microbial production of 1,3-propanediol (PDO), which is a versatile bulk chemical. PDO can be produced by microbial consortium with the advantages of high substrate tolerance and narrow by-product profile. In the present study, the effect of IL 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([Emim][TfO]) was evaluated on the capacity of PDO production from crude glycerol by microbial consortium DL38-BH. In the batch fermentation at 60 g/L crude glycerol and 10 g/L [Emim][TfO], the concentration and yield of PDO from glycerol increased from 23.14 g/L and 0.45 mol/mol to 31.17 g/L and 0.60 mol/mol, respectively. Our results showed that [Emim][TfO] decreased the ratio of intracellular NADH to NAD+ and increased the concentration of 3-HPA during batch fermentation. The activities of three key enzymes in glycerol metabolism were stimulated by [Emim][TfO] during the batch fermentation by microbial consortium DL38-BH. Compared to the control, the proportion of Klebsiella genus which could convert glycerol to PDO increased significantly from 79.19% to 89.49% and the other genera that did not produce PDO were dramatically decreased (P
- Subjects :
- Glycerol
0106 biological sciences
0301 basic medicine
Microbial Consortia
Ionic Liquids
Bioengineering
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
Klebsiella
010608 biotechnology
1,3-Propanediol
Mesylates
Chemistry
Imidazoles
Substrate (chemistry)
General Medicine
Microbial consortium
030104 developmental biology
Propylene Glycols
Yield (chemistry)
Biodiesel production
Ionic liquid
Industrial and production engineering
Biotechnology
Nuclear chemistry
Subjects
Details
- ISSN :
- 16157605 and 16157591
- Volume :
- 41
- Database :
- OpenAIRE
- Journal :
- Bioprocess and Biosystems Engineering
- Accession number :
- edsair.doi.dedup.....10d249bd6d8f83a3562c3ce51cf5bf1c