Back to Search
Start Over
Overlapping and Non-overlapping Functions of Condensins I and II in Neural Stem Cell Divisions
- Source :
- PLoS Genetics, PLoS Genetics, Vol 10, Iss 12, p e1004847 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- During development of the cerebral cortex, neural stem cells (NSCs) divide symmetrically to proliferate and asymmetrically to generate neurons. Although faithful segregation of mitotic chromosomes is critical for NSC divisions, its fundamental mechanism remains unclear. A class of evolutionarily conserved protein complexes, known as condensins, is thought to be central to chromosome assembly and segregation among eukaryotes. Here we report the first comprehensive genetic study of mammalian condensins, demonstrating that two different types of condensin complexes (condensins I and II) are both essential for NSC divisions and survival in mice. Simultaneous depletion of both condensins leads to severe defects in chromosome assembly and segregation, which in turn cause DNA damage and trigger p53-induced apoptosis. Individual depletions of condensins I and II lead to slower loss of NSCs compared to simultaneous depletion, but they display distinct mitotic defects: chromosome missegregation was observed more prominently in NSCs depleted of condensin II, whereas mitotic delays were detectable only in condensin I-depleted NSCs. Remarkably, NSCs depleted of condensin II display hyperclustering of pericentric heterochromatin and nucleoli, indicating that condensin II, but not condensin I, plays a critical role in establishing interphase nuclear architecture. Intriguingly, these defects are taken over to postmitotic neurons. Our results demonstrate that condensins I and II have overlapping and non-overlapping functions in NSCs, and also provide evolutionary insight into intricate balancing acts of the two condensin complexes.<br />Author Summary The cerebral cortex is built up of numerous neurons and cells supporting them, most of which originate from neural stem cells (NSCs). NSCs divide symmetrically to produce themselves and asymmetrically to generate neurons, and both types of divisions reply on faithful segregation of chromosomes into daughter cells. In the current study, we study the functions of evolutionarily conserved chromosome regulators, known as condensin I and condensin II, during development of the cerebral cortex in mice. We find that condensins I and II have both overlapping and non-overlapping functions in NSC divisions and survival: loss of either one of condensins causes distinct abnormalities in the process of chromosome segregation. Remarkably, loss of condensin II, but not of condensin I, also alters chromosome architecture during non-dividing stages. Our results demonstrate convincingly that an intricate balance between condensins I and II plays a crucial role in NSC divisions. It will be of great interest to test in the future whether such balancing acts of the two condensin complexes might be misregulated in tumorigenic NSCs that undergo uncontrolled cell divisions.
- Subjects :
- Cancer Research
Cell division
Nucleolus
Condensin
Apoptosis
Chromosome segregation
Mice
Neural Stem Cells
Pregnancy
Chromosome Segregation
Cell Cycle and Cell Division
Genetics (clinical)
Pericentric heterochromatin
Cells, Cultured
Genetics
Adenosine Triphosphatases
Mice, Knockout
biology
Chromosome Biology
Neural stem cell
Cell biology
DNA-Binding Proteins
Cell Processes
Female
Cell Division
Research Article
lcsh:QH426-470
Mitosis
macromolecular substances
Chromosomes
Molecular Genetics
Condensin complex
Animals
Mammalian Genetics
Molecular Biology
Ecology, Evolution, Behavior and Systematics
Biology and Life Sciences
Cell Biology
Chromatin Assembly and Disassembly
Embryo, Mammalian
Mice, Inbred C57BL
lcsh:Genetics
nervous system
Multiprotein Complexes
biology.protein
Animal Genetics
Developmental Biology
DNA Damage
Subjects
Details
- Language :
- English
- ISSN :
- 15537404 and 15537390
- Volume :
- 10
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- PLoS Genetics
- Accession number :
- edsair.doi.dedup.....10c623e7f3a55687178afa40f14c6c44