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Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells
Equine herpesvirus type 1 pUL56 modulates innate responses of airway epithelial cells
- Source :
- Virology. :76-86
- Publisher :
- Elsevier Inc.
-
Abstract
- Recently, the product of equine herpesvirus type 1 (EHV-1) ORF1, a homolog to HSV-1 pUL56, was shown to modulate MHC-I expression and innate immunity. Here, we investigated modulation of respiratory epithelial immunity by EHV-1 pUL56 and compared responses to those of PBMCs, which are important target cells that allow cell-associated EHV-1 viremia.The salient observations are as follows: (i) EHV-1 significantly down-modulated MHC-I and MHC-II expression in equine respiratory epithelial cells (ERECs). MHC-I expression remained unaffected in PBMCs and MHC-II expression was increased. (ii) Infection with an EHV-1 ORF1 deletion mutant partially restored MHC-I and MHC-II expression and altered IFN-alpha and IL-10 mRNA expression. (iii) Deletion of EHV-1 ORF1 also significantly increased chemokine expression and chemotaxis of monocytes and neutrophils in ERECs. Collectively, these results suggest a role for EHV-1 pUL56 in modulation of antigen presentation, cytokine expression and chemotaxis at the respiratory epithelium, but not in PBMC.
- Subjects :
- Chemokine
Equine herpesvirus 1
Antigen presentation
chemical and pharmacologic phenomena
Respiratory Mucosa
Viral Nonstructural Proteins
Major histocompatibility complex
Immunity
Virology
Animals
Horses
Innate immunity
Innate immune system
Equine herpesvirus-1
biology
Epithelial Cells
Chemotaxis
biology.organism_classification
Immunity, Innate
Host-Pathogen Interactions
Immunology
Respiratory epithelium
Leukocytes, Mononuclear
biology.protein
Horse Diseases
Chemokines
Herpesvirus 1, Equid
pUL56
Subjects
Details
- Language :
- English
- ISSN :
- 00426822
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....10be35ab1e185b7c58a1335cea7f3e3b
- Full Text :
- https://doi.org/10.1016/j.virol.2014.05.023