Back to Search
Start Over
A Clinical Application Study of Nanostructured Substrates for the Detection of Circulating Tumor Cells
- Source :
- Clinical Laboratory. 65
- Publication Year :
- 2019
- Publisher :
- Clinical Laboratory Publications, 2019.
-
Abstract
- BACKGROUND Circulating tumor cell (CTC) isolation methods based on nanostructured substrates can be used to isolate tumor cells from peripheral blood. This study aimed to validate the clinical application of our method and determine the appropriate diagnostic critical value. METHODS AFM was used to detect the surface roughness of nanostructured substrates. Cell lines and blood samples were used to verify CTC isolation methods. The ROC curve and AUC were used to evaluate the diagnostic value of CTC numbers. RESULTS First, AFM, cell binding yields, and tumor cell detection rate from blood showed that NS has a potential for cell adsorption. Then, the CTC detection method was verified by using cell lines and blood samples. The number of CTCs in patients with cancers or metastases were significantly greater than those of patients without cancers. Then, the ROC curves and AUC showed that this method had a medium diagnostic value. CONCLUSIONS Isolating CTCs based on nanostructured substrates was appropriate for the clinical diagnosis of tumors, and samples with more than 1.5 CTCs/1 mL blood could be identified as CTC-positive.
- Subjects :
- Male
Receiver operating characteristic
Chemistry
Atomic force microscopy
Cell
Middle Aged
Neoplastic Cells, Circulating
General Biochemistry, Genetics and Molecular Biology
Peripheral blood
Blood Cell Count
Nanostructures
Cell binding
medicine.anatomical_structure
Circulating tumor cell
Case-Control Studies
Neoplasms
Clinical diagnosis
MCF-7 Cells
Cancer research
medicine
Humans
Female
In patient
Subjects
Details
- ISSN :
- 14336510
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Clinical Laboratory
- Accession number :
- edsair.doi.dedup.....10b3aaa6bf4db95ca4d43d9db6b8dab6