Cross reactions of a panosyl-azoprotein conjugate with antipneumococcal horse sera

Panosyl-BSA, a synthetic sugar-azoprotein conjugate consisting of the trisaccharide panose (glucosyl α-(1,6)-glucosyl-α(1,4) glucose) coupled to bovine serum albumin by an azphenyl linkage, cross precipitates antibody from types II and XX antipneumococcal horse sera. Hapten inhibition indicates that these cross reactions of synthetic antigen are mediated by the α-isomaltosyl moiety of the introduced panosyl haptenic grouping. While interaction of conjugate with horse anti-XX involves a fraction of antibody directed against an α-isomaltosyl end group, interaction with horse anti-II involves a fraction of antibody directed against the α-isomaltosyl end group. Purified fractions of antibody, cross reactive with panosly-BSA were specifically from antipneumococcal horse sera and their behavior with homologous antigen examined and compared with unfractionated serum.