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Native DIGE proteomic analysis of mitochondria from substantia nigra and striatum during neuronal degeneration and its compensation in an animal model of early Parkinson's disease
- Source :
- Archives of physiology and biochemistry. 122(5)
- Publication Year :
- 2016
-
Abstract
- Cause of Parkinson's disease (PD) is still not understood. Motor symptoms are not observed at early stages of disease due to compensatory processes. Dysfunction of mitochondria was indicated already at preclinical PD. Selective toxin 6-OHDA was applied to kill dopaminergic neurons in substantia nigra and disturb neuronal transmission in striatum. Early phase of active degeneration and later stage, when surviving cells adapted to function normally, were analysed. 2D BN/SDS difference gel electrophoresis (DIGE) of mitochondrial proteome enabled to point out crucial processes involved at both time-points in dopaminergic structures. Marker proteins such as DPYSL2, HSP60, ATP1A3, EAAT2 indicated structural remodelling, cytoskeleton rearrangement, organelle trafficking, axon outgrowth and regeneration. Adaptations in dopaminergic and glutamatergic neurotransmission, recycling of synaptic vesicles, along with enlargement of mitochondria mass were proposed as causative for compensation. Changed expression of carbohydrates metabolism and oxidative phosphorylation proteins were described, including their protein-protein interactions and supercomplex assembly.
- Subjects :
- 0301 basic medicine
Male
Proteomics
Parkinson's disease
Proteome
Physiology
Substantia nigra
Striatum
Mitochondrion
Biology
Synaptic vesicle
Mitochondrial Proteins
03 medical and health sciences
0302 clinical medicine
Physiology (medical)
medicine
Animals
Electrophoresis, Gel, Two-Dimensional
Rats, Wistar
Dopaminergic Neurons
Dopaminergic
Neurodegeneration
Parkinson Disease
General Medicine
medicine.disease
Corpus Striatum
Cell biology
Mitochondria
Rats
Substantia Nigra
Disease Models, Animal
030104 developmental biology
nervous system
Nerve Degeneration
Collapsin response mediator protein family
Neuroscience
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 17444160
- Volume :
- 122
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Archives of physiology and biochemistry
- Accession number :
- edsair.doi.dedup.....10b14383b761586724f391c39b1a1e53