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Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration
- Source :
- Cicek, R, Tokarz, D, Steup, M, Tetlow, I J, Emes, M J, Hebelstrup, K H, Blennow, A & Barzda, V 2015, ' Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration ', Biomedical Optics Express, vol. 6, no. 10, pp. 3694-3700 . https://doi.org/10.1364/BOE.6.003694, Cisek, R, Tokarz, D, Steup, M, Tetlow, I J, Emes, M J, Hebelstrup, K, Blennow, A & Barzda, V 2015, ' Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration ', Biomedical Optics Express, vol. 6, no. 10, pp. 3694-3700 . https://doi.org/10.1364/BOE.6.003694
- Publication Year :
- 2015
-
Abstract
- Second harmonic generation (SHG) microscopy is employed to study changes in crystalline organization due to altered gene expression and hydration in barley starch granules. SHG intensity and susceptibility ratio values (R'(SHG)) are obtained using reduced Stokes-Mueller polarimetric microscopy. The maximum R'(SHG) values occur at moderate moisture indicating the narrowest orientation distribution of nonlinear dipoles from the cylindrical axis of glucan helices. The maximum SHG intensity occurs at the highest moisture and amylopectin content. These results support the hypothesis that SHG is caused by ordered hydrogen and hydroxyl bond networks which increase with hydration of starch granules. (C) 2015 Optical Society of America
- Subjects :
- chemistry.chemical_classification
animal structures
Starch
Analytical chemistry
food and beverages
Second-harmonic generation
Nanotechnology
Second Harmonic Generation Microscopy
Biology
Article
Atomic and Molecular Physics, and Optics
chemistry.chemical_compound
chemistry
Amylopectin
Microscopy
X-ray crystallography
Ultrastructure
Institut für Biochemie und Biologie
Biotechnology
Glucan
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Cicek, R, Tokarz, D, Steup, M, Tetlow, I J, Emes, M J, Hebelstrup, K H, Blennow, A & Barzda, V 2015, ' Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration ', Biomedical Optics Express, vol. 6, no. 10, pp. 3694-3700 . https://doi.org/10.1364/BOE.6.003694, Cisek, R, Tokarz, D, Steup, M, Tetlow, I J, Emes, M J, Hebelstrup, K, Blennow, A & Barzda, V 2015, ' Second harmonic generation microscopy investigation of the crystalline ultrastructure of three barley starch lines affected by hydration ', Biomedical Optics Express, vol. 6, no. 10, pp. 3694-3700 . https://doi.org/10.1364/BOE.6.003694
- Accession number :
- edsair.doi.dedup.....10b09b52ab7f0285dca7ceceef879cf9