Additional file 2 of Insulin-like growth factor-1 receptor controls the function of CNS-resident macrophages and their contribution to neuroinflammation

Additional file 2. Transcriptomic changes in microglia and BAMs upon IGF-1R deletion. A) Sorting strategy of microglia (CD45+CD11b+P2ry12+tdTomato+) and BAMs (CD45+CD11b+P2ry12-CD206+tdTomato+). Cells were sorted from the brain of IGF-1RKO-tdTomato and IGF-1RWT-tdTomato mice directly into RNA protect buffer. B) Venn diagram showing the concordance of gene expression differences in microglia and BAMs from IGF-1RKO-tdTomato and IGF-1RWT-tdTomato mice C) Functional enrichment analysis of the microglia from IGF-1RKO-tdTomato and IGF-1RWT-tdTomato mice using all pathways from the Gene Ontology database. Left, pathways upregulated in IGF-1RKO-tdTomato mice; right, pathways downregulated in IGF-1RKO-tdTomato mice.