Establishment and characterization of an oral squamous carcinoma cell line from a patient with no apparent habits associated with oral cancer

Introduction: We have established a cell line ORL-48(T) from a surgically resected specimen of an untreated primary human oral squamous cell carcinoma of the mandible.This patient did not appear to have any oral habits which have been reported to be associated with oral cancer Materials and Method: The in vitro growth characteristics, epithelial origin, in vitro anchorage independency, HPV infection, microsatellite instability status, karyotype and the status of various cell cycle regulators and gatekeepers of the ORL-48(T) cell line were investigated. Results: The ORL-48(T) cell line is immortal, 3T3- independent and grew as a monolayer with the doubling time of 48h. Immunohistochemistry staining of cytokeratins confirmed the cell line is of epithelial origin. Soft agar assays demonstrated that ORL-48(T) expressed a low degree of anchorage independency (CFE