Supplemental Figure S3 from Targeting Acidity in Pancreatic Adenocarcinoma: Multispectral Optoacoustic Tomography Detects pH-Low Insertion Peptide Probes In Vivo

Supplemental Figure S3: Elliptical 3.5 mm2 ROIs were drawn based upon anatomical location and this size was maintained for relative quantitation of signal accumulation in the pancreas tumor, kidney, and liver. Because the liver and kidney are more easily identified in separate slices, two slices are shown which also contain the pancreas tumor. The location of orthotopic tumors was identified based upon the presence of deoxy-hemoglobin and relative location of the spleen and kidney as indicated by a yellow ellipse (A-B). Due to the location of the pancreas tumor and to prevent errors in organ identification, elliptical 3.5 mm2 ROIs were also drawn in the (A) uninvolved liver (red ellipse) and (B) opposite kidney (white ellipse). The 3.5 mm2 ellipse size was maintained among all ROIs in all mice and for all organs, but ROIs were rotated as needed based upon deoxy-hemoglobin localization to ensure that the probe accumulation within the pancreas tumors as accurate.