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Dopamine transporter synthesis and degradation rate in rat striatum and nucleus accumbens using RTI-76
- Source :
- Neuropharmacology. 39:578-585
- Publication Year :
- 2000
- Publisher :
- Elsevier BV, 2000.
-
Abstract
- Intracerebroventricular injections of the irreversible dopamine transporter (DAT) inhibitor, RTI-76 [3beta-(3-p-chlorophenyl) tropan-2beta-carboxylic acid p-isothiocyanatophenylethyl ester hydrochloride], decreased DAT binding in both the striatum and nucleus accumbens as measured by both [3H]GBR12935 and by [3H]WIN35,428. This decrease was dose-related, with 100 nmol RTI-76 producing approximately a 50% decrease in both regions. The maximal inhibition of DAT binding was observed 24 h after RTI-76 injection, and binding was fully restored 7 days after injection. The DAT protein half-life determined under these conditions was about 2 days. [3H]Nisoxetine binding at norepinephrine transporters in the cortex was not altered by RTI-76 administration at any time point or dose examined.
- Subjects :
- Male
medicine.medical_specialty
Dopamine
Nerve Tissue Proteins
Striatum
Nucleus accumbens
Ligands
Nucleus Accumbens
Piperazines
Rats, Sprague-Dawley
Norepinephrine
Cellular and Molecular Neuroscience
Cocaine
Dopamine Uptake Inhibitors
Fluoxetine
Internal medicine
medicine
Animals
Injections, Intraventricular
Dopamine transporter
Pharmacology
Dopamine Plasma Membrane Transport Proteins
Membrane Glycoproteins
Norepinephrine Plasma Membrane Transport Proteins
Symporters
biology
Chemistry
Membrane Transport Proteins
Transporter
Nisoxetine
Corpus Striatum
Rats
Cortex (botany)
Endocrinology
biology.protein
Carrier Proteins
human activities
Protein Binding
Tropanes
medicine.drug
Subjects
Details
- ISSN :
- 00283908
- Volume :
- 39
- Database :
- OpenAIRE
- Journal :
- Neuropharmacology
- Accession number :
- edsair.doi.dedup.....105c7410f3d21d09247588bc925f0627